EpiMark核小体组装试剂盒                                                            货号：A-E5350S

EpiMark® Nucleosome Assembly Kit

背景资料：The EpiMark® Nucleosome Assembly Kit is used to make unmodified recombinant human nucleosomes with user-supplied DNA or the provided control DNA. Purified recombinant human Histone H2A/H2B Dimer and Histone H3.1/H4 Tetramer are mixed with DNA at 2 M NaCl. To generate nucleosomes, the salt concentration is lowered by dilution or dialysis allowing each histone tetramer to associate with two histone dimers and form the histone octamer on the DNA (1,2). A method for assaying nucleosome formation by gel shift assay is included. These nucleosomes may serve as better substrates for enzymes that are inactive on the DNA or one of the core histones alone (3,4). Each reaction creates nucleosomes from ~1 µg of 208 bp DNA and may be scaled depending on the experiment.

产品描述：A gel shift assay allows visualization of complex formation when various ratios of Octamer* to DNA are used to form mononucleosomes using the provided nucleosome control DNA. When assembly reactions are run on 6% polyacrylamide gel in 0.5X TBE, a characteristic shift occurs from 208 bp to approximately 700 bp.

产品特点：

1.Highly pure, recombinant system；

2.Components stable for one year ；

3.Pre-formed Histone Dimer and Tetramer complexes simplify Octamer formation;

4.Dilution protocol only requires a few hours for assembly;

5.ChIP assay ;HAT assay (Histone Acetyltransferase Assay);Enzymatic modification assay, (e.g., methylation) 。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

定购信息：