EpiMark 5-hmC和5-mC 分析试剂盒                                                             货号：A-E3317S

EpiMark® 5-hmC and 5-mC Analysis Kit

背景资料：5-methylcytosine (5-mC) is the predominant epigenetic mark in mammalian genomic DNA. 5-hydroxymethylcytosine (5-hmC) is a newly discovered epigenetic modification that is presumably generated by oxidation of 5-mC by the TET family of cytosine oxygenases(1)()..

Techniques exist that can identify 5-mC in genomic DNA, but the most commonly used method, bisulfite sequencing, is laborious and cannot distinguish between 5-mC from 5-hmC(2).

产品描述：The EpiMark® 5-hmC and 5-mC Analysis Kit can be used to analyze and quantitate 5-methylcytosine and 5-hydroxymethylcytosine within a specific locus. The kit distinguishes 5-mC from 5-hmC by the addition of glucose to the hydroxyl group of 5-hmC via an enzymatic reaction utilizing T4 β-glucosyltransferase (T4-BGT). When 5-hmC occurs in the context of CCGG, this modification converts a cleavable MspI site to a noncleavable one.

产品特点：

1.Complete conversion of 5-hmC to glucosylated 5-hmC in DNA；

2.Discrimination between 5-mC and 5-hmC in CCGG sequences using enzymatic；

3.Digestion and PCR amplification;

4.Relative quantitation of 5-mC and 5-hmC. Easy-to-use protocol。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

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