游离环状DNA提取试剂盒（磁珠法）又名：ccfDNA提取试剂盒（磁珠法）                      货号：A-P-1064

EpiQuik™ Circulating Cell-Free DNA Isolation Kit

背景资料：Genetic and epigenetic analysis of circulating cell-free DNA (ccfDNA) in plasma/serum or other body fluids provides unique opportunities for early detection of a wide range of clinical disorders such as cancer, autoimmune disease, infection and fetal disorders. It was demonstrated that ccfDNA of clinical importance occurs predominantly as fragments of approximately 170 bases from mononucleosomes with a smaller proportion as fragments of 360 bases from di-nucleosomes [1,2 ]. Such nucleosomal complexes are released into blood circulation during apoptotic cell death and will be increased under various pathological circumstances such as inflammation, pulmonary embolism, autoimmune disease, and cancer [3,4]. It is also shown that using ccfDNA from such nucleosomal complexes for genetic or epigenetic analysis provides better and more accurate identification of physiological and pathological status [5]. There are several methods currently being used for ccfDNA isolation from plasma and serum. All of these methods are based on capture of DNA by silicone column binding or phenol-chloroform separation. The DNA isolated by these methods contains both ccfDNA and non-ccfDNA, which may affect the accuracy of downstream analysis. To address these problems, Epigentek offers the EpiQuik™ Circulating Cell-Free DNA Isolation Kit for ccfDNA isolation.

产品描述：The EpiQuik™ Circulating Cell-Free DNA Isolation Kit contains all components which have been optimized for the simple and rapid isolation of small size nucleosomal DNA from plasma/serum. The mono- and di-nucleosomal complexes are efficiently captured via size-fractionation magnetic beads (ccfDNA Capture Beads) by applying the beads to a magnetic field (EpiMag™ HT (96-Well) Magnetic Separator or similar). The captured nucleosomal DNA is then enzymatically released, and purified using MQ Binding Beads by simply washing the beads. The purified ccfDNA is then eluted from the beads for immediate use or storage.

产品特点：

1·Uses innovative magnetic bead based size-fractionation technology for selective isolation of circulating cell-free DNA from plasma/serum that is mainly 170 bps in size. The isolated DNA can be directly used for both qPCR and NGS DNA library preparation；

2·Fast and straightforward procedure can be finished within 2 hours. No gels, columns or centrifugation is needed；

3.Efficient removal of proteins, salts, nucleases, PCR inhibiting substances, and other impurities such as polysaccharides, polyphenols and lipids；

4.Sensitive and efficient DNA capture enables successful isolation with high recovery (>80% of input monoucleosomal DNA), even when the quantities of starting material are limited (as low as 0.1 ml)；

5.Manual and automation friendly – Scalable for single tube or 96-well plate formats。

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注释	游离环状DNA提取试剂盒（磁珠法）(A-P-1064)步骤流程	ccfDNA高得率:使用游离环状DNA提取试剂盒（磁珠法）从0.5ml的血浆，不同数量的单核小体HeLa中所得DNA.提到DNA采用荧光法和相同数量的样本所得的标准品进行定量分析。	高回收的ccfDNA被生物分析仪分析得到证实：从0.5ml的血浆，不同数量的单核小体HeLa中所得DNA.:红色：200ng;深蓝和绿色：500ng;天空蓝色：500ng未纯化的Hela单核小体作为对照。提取到的DNA片段大小在：170bps。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

产品名称	规格	操作手册	货号	询价
游离环状DNA提取试剂盒（磁珠法）	25 次	英文PDF-中文PDF	A-P-1064-25
游离环状DNA提取试剂盒（磁珠法）	50 次	英文PDF-中文PDF	A-P-1064-50
DNA甲基化极易修饰试剂盒	50次	英文PDF-中文PDF	A-P-1054
96孔DNA甲基化极速修饰试剂盒（磁珠法）	96次	英文PDF-中文PDF	A-P-1050-096
96孔DNA甲基化极速修饰试剂盒（磁珠法）	192次	英文PDF-中文PDF	A-P-1050-192
抗人C5a/C5a desArg单克隆抗体(克隆号2942)	100ug	PDF	C-HM2078
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记	100ug	PDF	C-HM2022F
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记	50ug	PDF	C-HM2008
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记	50ug	PDF	C-HM2006
抗小鼠TREM-2单克隆抗体(克隆号6E9)	100ug	PDF	C-HM1129F
m6A RNA甲基化定量检测试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-9005-96
RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-P-9003-050
EZ RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-R5001

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1036-96
DNA羟甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1037-96
DNA甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	英文PDF--中文PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	英文PDF--中文PDF	A-P-1014B-96

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