亚硫酸盐转化试剂盒 货号：E-55016
背景资料：Active Motif's Bisulfite Conversion Kit simplifies the analysis of DNA methylation by providing optimized conversion reagents and an easy-to-use protocol. DNA of interest is rapidly heat denatured in a thermocycler in the presence of the bisulfite conversion reagent and a DNA denaturant. The conversion reaction can be performed in as little as 1.5 hours with a 99% conversion efficiency of unmethylated cytosines to uracils. After DNA conversion, the sample is added to the included DNA purification columns, and a simple, on-column desulfonation is performed. Ready-to-use DNA is then eluted from the columns. For your convenience, a positive control conversion-specific PCR primer pair that is specific for bisulfite converted human and mouse DNA is included. Because the primer pair produces a PCR product only if conversion has occurred, you can validate the success of the conversion reaction before spending extra time and money on sequencing..
Active Motif also offers it's original MethylDetector™ Bisulfite Modification Kit. This assay performs bisulfite conversion of DNA using the same principle as the Bisulfite Conversion Kit, but is optimized for use with a 5 hour conversion reaction
产品描述：DNA methylation is an essential epigenetic modification that plays a key role in transcriptional regulation and assures the proper regulation of gene expression and stable gene silencing in normal cells. In methylation, a methyl group is added to the fifth-carbon of cytosine in a CpG dinucleotide by a DNA methyltransferase. As aberrant methylation is prevalent in many human cancers, and because methylation is also involved in embryonic development and cell cycle regulation, alterations in the methylation status of DNA can serve as promising indicators for disease diagnosis and prognosis..
The bisulfite conversion reaction was first described in the early 1970s to distinguish between cytosine and 5-methylcytosine (5-mC) in DNA1,2. It is now known that both 5-methylcytosine and 5-hydroxymethylcytosine (5-hmC) remain unchanged during conversion and, therefore, this method cannot be used to distinguish between the different methyl modifications. In the bisulfite conversion reaction, DNA is first treated with sodium bisulfite to convert cytosine residues into a cytosine-bisulfite derivative in denatured (single-stranded) DNA. This reaction is reversible, with the forward reaction being favored by high temperature and low pH. The second step is an irreversible hydrolytic deamination of the cytosine-bisulfite derivative that results in a uracil-bisulfite derivative. This reaction is also favored by low pH. The final step involves the desulfonation of the uracil-bisulfite derivative to uracil under high pH conditions. Only unmethylated cytosines are susceptible to the bisulfite reaction, therefore 5-mC and 5-hmC remain the unchanged. Following bisulfite conversion, the DNA is often amplified by PCR where the uracils are converted to thymines
产品使用：Active Motif's Bisulfite Conversion Kit simplifies the analysis of DNA methylation by providing optimized conversion reagents, an easy-to-use protocol and a positive control PCR primer set to validate results. The conversion reaction can be performed in as little as 1.5 hours with a 99% conversion efficiency. For your convenience, a conversion-specific PCR primer pair is included to validate the success of the bisulfite conversion before starting sequencing or other analysis methods. The positive control primer pair is specific for bisulfite-converted human and mouse DNA and will only produce a 220 bp PCR product if the conversion reaction was successful。
产品应用：PCR results of human (A) and mouse (B) genomic DNA that was either bisulfite converted (Bis.) according to the Bisulfite Conversion Kit assay protocol or untreated (Untr.), then amplified using the included positive control conversion-specific PCR primer pair. The primer pair is specific towards bisulfite converted DNA and, therefore, a 220 bp PCR amplicon is present in the bisulfite treated DNA samples and not the untreated DNA samples.
保存建议：The Bisulfite Conversion Kit is shipped at room temperature and can be stored at 4°C prior to first use. Then, we recommend storing each component at the temperatures listed in the table below. Components are guaranteed stable for 6 months from the date of receipt when stored properly.
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 亚硫酸盐转化试剂盒 |
50rxns |
PDF |
E-55016 |
 |
| 重组 DNMT1 蛋白, active |
10ug |
PDF |
E-31335 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
100ug |
PDF |
E-61495 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
10ug |
PDF |
E-61496 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
100ul |
PDF |
E-61323 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
10ul |
PDF |
E-61324 |
|
| DNMT3A 抗体 (mAb) |
200ug |
PDF |
E-39206-200 |
|
| DNMT3A 抗体 (mAb) |
100ug |
PDF |
E-39206-100 |
|
| 3-Methylcytosine (3-mC) 抗体 |
100ug |
PDF |
E-61111 |
|
| 3-Methylcytosine (3-mC) 抗体 |
10ug |
PDF |
E-61112 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
100ug |
PDF |
E-A-9000-100 |
| CRISPR/Cas9 单克隆抗体【7A9】 |
50 ug |
PDF |
E-A-9000-50 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
10 ug |
PDF |
E-A-9000-10 |
|
| CRISPR/Cas9 多克隆抗体 |
100ug |
PDF |
E-A-1111-100
|
|
| CRISPR/Cas9 多克隆抗体 |
50 ug |
PDF |
E-A-1111-050
|
|
| CRISPR/Cas9 多克隆抗体 |
10 ug |
PDF |
E-A-1111-010
|
|
| APC标记大鼠抗小鼠CD90.2/Thy-1.2抗体 |
0.1mg |
PDF |
E-1750-11 |
|
| PE标记仓鼠抗小鼠CD69/VEA抗体 |
0.2mg |
PDF |
E-1715-09L |
|
| 低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体 |
0.5mg |
PDF |
E-1531-14 |
|
| 生物素标记山羊抗I型胶原蛋白抗体 |
0.2mg |
PDF |
E-1310-08 |
|
| 兔抗人DR5抗体 |
0.1mg |
PDF |
E-6600-01 |
|
| SEPH标记山羊抗s-tag标签抗体 |
1.0ml |
PDF |
E-6500-25 |
|
| 抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF--中文PDF |
A-P-1050-192 |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
