MODified™组蛋白多肽分析试剂盒 货号：E-13005
MODified™ Histone Peptide Array
背景资料：The MODified Histone Peptide Array* is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM). The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation, and citrullination modifications on the N-terminal tails of histones H2A, H2B, H3 and H4. Each peptide array contains 384 unique histone modification combinations in duplicate, including up to four separate modifications on the same 19mer peptide, offering the most extensive coverage available for commercial arrays of similar format. This extensive coverage of histone modifications enables the study not only of individual sites, but also the effects of neighboring modifications on recognition and binding. The unique synthesis and conjugation methods ensure greater than 95% purity of bound peptides and high peptide density at each spot, which is advantageous for analysis of interaction sites with low binding constants. The simple array protocol works like a Western blot; no special equipment needed.
产品描述：The MODified Histone Peptide Array is designed to study the binding specificity of antibodies, enzymes and proteins. The arrays are glass slides that contain up to four histone post-translational modifications per peptide to allow for analysis of individual histone modifications as well as the effects of neighboring modifications. The MODified Array Labeling Kit and the MODified Protein Domain Binding Kit are complementary products containing the buffers and reagents required to process the peptide array for chemiluminescent detection. Please see the table below for the combination of reagents recommended for each application.
产品使用：Active Motif uses the Histone Peptide Array to help validate the binding specificity of our histone modification antibodies. Select antibody images and graphs are available below. To see a complete list of all the Active Motif antibodies that have been tested using the MODified™ Histone Peptide Array, please follow the link to array tested
产品应用：.
How do the MODified™ Histone Peptide Arrays Work?
To generate MODified Arrays, Active Motif synthesizes a series of 19mer histone H2A, H2B, H3 and H4 peptides, each of which may contain as many as four modifications per peptide, on a modified cellulose support which can be dissolved after synthesis. The solutions of peptides covalently linked to macromolecular cellulose are then spotted in duplicate onto a glass slide as a three-dimensional layer. This high peptide density in the spots is advantageous for identifying protein-interaction sites with low binding constants. The peptide coated slides contain 768 spots (384 modification combinations in duplicate) and are offered as single arrays (Catalog No. 13001) or as a pack of five arrays (Catalog No. 13005).
The MODified Histone Peptide Arrays can be used to screen antibodies, enzymes and proteins for cross reactivity or binding interactions with histones and their post-translational modifications. Following a blocking step, the array is incubated with either antibodies, proteins or enzymes for one hour, washed and then incubated for one hour with either a horseradish peroxidase (HRP) or alkaline phosphatase (AP) conjugated secondary antibody. Developing solution is then added and the image is captured using film or a CCD camera; no special equipment is needed. The MODified Histone Peptide Arrays can also be analyzed using the LI-COR® Odyssey® infrared imaging system and infrared detection reagents
保存建议：The MODified Histone Peptide Arrays can be stored at 4°C for up to three months. For long-term storage of up to six months, we recommend keeping the arrays at -20°C. The arrays are available individually or in packs of 5 arrays..
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| MODified™组蛋白多肽分析试剂盒 |
5 arrays |
PDF |
E-13005 |
 |
| MODified™组蛋白多肽分析试剂盒 |
1 arrays |
PDF |
E-13001 |
|
| 重组 DNMT1 蛋白, active |
10ug |
PDF |
E-31335 |
|
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100ug |
PDF |
E-61495 |
|
| 6-Methyladenosine (6-meA) 抗体 (pAb) |
10ug |
PDF |
E-61496 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
100ul |
PDF |
E-61323 |
|
| DNMT3A dimethyl Lys44 抗体 (pAb) |
10ul |
PDF |
E-61324 |
|
| DNMT3A 抗体 (mAb) |
200ug |
PDF |
E-39206-200 |
|
| DNMT3A 抗体 (mAb) |
100ug |
PDF |
E-39206-100 |
|
| 3-Methylcytosine (3-mC) 抗体 |
100ug |
PDF |
E-61111 |
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| 3-Methylcytosine (3-mC) 抗体 |
10ug |
PDF |
E-61112 |
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100ug |
PDF |
E-A-9000-100 |
| CRISPR/Cas9 单克隆抗体【7A9】 |
50 ug |
PDF |
E-A-9000-50 |
|
| CRISPR/Cas9 单克隆抗体【7A9】 |
10 ug |
PDF |
E-A-9000-10 |
|
| CRISPR/Cas9 多克隆抗体 |
100ug |
PDF |
E-A-1111-100
|
|
| CRISPR/Cas9 多克隆抗体 |
50 ug |
PDF |
E-A-1111-050
|
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| CRISPR/Cas9 多克隆抗体 |
10 ug |
PDF |
E-A-1111-010
|
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0.1mg |
PDF |
E-1750-11 |
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0.2mg |
PDF |
E-1715-09L |
|
| 低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体 |
0.5mg |
PDF |
E-1531-14 |
|
| 生物素标记山羊抗I型胶原蛋白抗体 |
0.2mg |
PDF |
E-1310-08 |
|
| 兔抗人DR5抗体 |
0.1mg |
PDF |
E-6600-01 |
|
| SEPH标记山羊抗s-tag标签抗体 |
1.0ml |
PDF |
E-6500-25 |
|
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100ug |
PDF |
C-HM1129F |
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| 96孔DNA甲基化极速修饰试剂盒（磁珠法） |
192次 |
英文PDF--中文PDF |
A-P-1050-192 |
|
| m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
| RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
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| EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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