MODified™蛋白质结构域结合试剂盒 货号:E-13007
MODified™ Protein Domain Binding Kit
背景资料:The MODified Histone Peptide Array* is a valuable research tool that can be used to screen antibodies, enzymes and proteins for cross-reactivity or binding interactions with histones and their post-translational modifications (PTM). The MODified Histone Peptide Arrays screen 59 acetylation, methylation, phosphorylation, and citrullination modifications on the N-terminal tails of histones H2A, H2B, H3 and H4. Each peptide array contains 384 unique histone modification combinations in duplicate, including up to four separate modifications on the same 19mer peptide, offering the most extensive coverage available for commercial arrays of similar format.
产品描述:This extensive coverage of histone modifications enables the study not only of individual sites, but also the effects of neighboring modifications on recognition and binding. The unique synthesis and conjugation methods ensure greater than 95% purity of bound peptides and high peptide density at each spot, which is advantageous for analysis of interaction sites with low binding constants. The simple array protocol works like a Western blot; no special equipment needed.
产品使用:Active Motif uses the Histone Peptide Array to help validate the binding specificity of our histone modification antibodies. Select antibody images and graphs are available below. To see a complete list of all the Active Motif antibodies that have been tested using the MODified™ Histone Peptide Array, please follow the link to array tested
产品应用:.
How do the MODified™ Histone Peptide Arrays Work?
Antibodies, Enzymes and Protein Domains – Active Motif offers a broad selection of histone modification antibodies, HAT, HDAC, HMT or HDMproteins and bromodomains that can be utilized in combination with the MODified Histone Peptide Array.
保存建议:The MODified Protein Domain Binding Kits are shipped on dry ice and contain reagents with multiple storage temperatures inside. Please store each component at the temperature indicated below. This kit includes the following components:
Recombinant His-G9a protein; Store at -80°C
Blocking Buffer AM2; Store at -20°C
Interaction Buffer AM1; Store at -20°C
1 M DTT; Store at -20°C
Anti-His6-tag antibody; Store at -20°C
10X Wash Buffer AM4; Store at 4°C
Anti-mouse HRP-conjugated secondary antibody; Store at 4°C
ECL Reagent A; Store at 4°C
ECL Reagent B; Store at 4°C.
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
MODified™蛋白质结构域结合试剂盒 |
5 rxns |
PDF |
E-13007 |
|
MODified™组蛋白多肽分析试剂盒 |
5 arrays |
PDF |
E-13005 |
|
MODified™组蛋白多肽分析试剂盒 |
1 arrays |
PDF |
E-13001 |
|
重组 DNMT1 蛋白, active |
10ug |
PDF |
E-31335 |
|
6-Methyladenosine (6-meA) 抗体 (pAb) |
100ug |
PDF |
E-61495 |
|
6-Methyladenosine (6-meA) 抗体 (pAb) |
10ug |
PDF |
E-61496 |
|
DNMT3A dimethyl Lys44 抗体 (pAb) |
100ul |
PDF |
E-61323 |
|
DNMT3A dimethyl Lys44 抗体 (pAb) |
10ul |
PDF |
E-61324 |
|
DNMT3A 抗体 (mAb) |
200ug |
PDF |
E-39206-200 |
|
DNMT3A 抗体 (mAb) |
100ug |
PDF |
E-39206-100 |
|
3-Methylcytosine (3-mC) 抗体 |
100ug |
PDF |
E-61111 |
|
3-Methylcytosine (3-mC) 抗体 |
10ug |
PDF |
E-61112 |
|
CRISPR/Cas9 单克隆抗体【7A9】 |
100ug |
PDF |
E-A-9000-100 |
CRISPR/Cas9 单克隆抗体【7A9】 |
50 ug |
PDF |
E-A-9000-50 |
|
CRISPR/Cas9 单克隆抗体【7A9】 |
10 ug |
PDF |
E-A-9000-10 |
|
CRISPR/Cas9 多克隆抗体 |
100ug |
PDF |
E-A-1111-100
|
|
CRISPR/Cas9 多克隆抗体 |
50 ug |
PDF |
E-A-1111-050
|
|
CRISPR/Cas9 多克隆抗体 |
10 ug |
PDF |
E-A-1111-010
|
|
APC标记大鼠抗小鼠CD90.2/Thy-1.2抗体 |
0.1mg |
PDF |
E-1750-11 |
|
PE标记仓鼠抗小鼠CD69/VEA抗体 |
0.2mg |
PDF |
E-1715-09L |
|
低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体 |
0.5mg |
PDF |
E-1531-14 |
|
生物素标记山羊抗I型胶原蛋白抗体 |
0.2mg |
PDF |
E-1310-08 |
|
兔抗人DR5抗体 |
0.1mg |
PDF |
E-6600-01 |
|
SEPH标记山羊抗s-tag标签抗体 |
1.0ml |
PDF |
E-6500-25 |
|
抗小鼠TREM-2单克隆抗体(克隆号6E9) |
100ug |
PDF |
C-HM1129F |
|
96孔DNA甲基化极速修饰试剂盒(磁珠法) |
192次 |
英文PDF--中文PDF |
A-P-1050-192 |
|
m6A RNA甲基化定量检测试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-9005-96 |
|
RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-P-9003-050 |
|
EZ RNA甲基化修饰试剂盒 |
50 次 |
英文PDF--中文PDF |
A-R5001 |
|
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |