总组蛋白H3K27乙酰化定量检测试剂盒（比色法）                                                      货号：A-P-4059

EpiQuik Global Acetyl Histone H3K27 Quantification Kit (Colorimetric)

背景资料：Acetylation of histones, including histone H3, has been involved in the regulation of chromatin structure and recruitment of transcription factors to the gene promoters. Histone acetyltransferases (HATs) and histone deacetylases (HDACs) play a critical role in the control of histone H3 acetylation at multiple sites. Histone H3 at lysine 27 (H3-K27) is one of the primary acetylated sites of histone H3. Acetylation of histone H3-K27 is tightly involved in cell cycle regulation, cell proliferation, and apoptosis; acetylation of histone H3-K27 is also an active marker. For example, loss of Polycomb Repressive Complex 2 (PRC2) activity results in a global increase in H3K27 acetylation suggesting that PRC2 represses transcription by preventing the binding of acetyltrasferases to K27 position. H3-K27 acetylation shows a robust peak at the transcription start site (TSS) of active and poised genes. The balance between histone H3-K27 acetylation and methylation is important for the establishment of specific chromatin structures. An imbalance in the equilibrium of histone H3 acetylation, including K27 acetylation, has been associated with tumorigenesis and cancer progression. Histone H3-K27 acetylation may be increased by inhibition of HDACs and decreased by HAT inhibition. Thus, quantitative detection of global acetyl histone H3-K27 would provide useful information for better understanding epigenetic regulation of gene activation and for developing HAT or HDAC-targeted drugs. The EpiQuik™ Global Acetyl Histone H3K27 Quantification Kit (Colorimetric) provides a tool for measuring global acetylation of histone H3-K27。

产品描述：This kit is designed for measuring global histone H3-K27 acetylation. In an assay with this kit, the acetyl histone H3 at lysine 27 is captured to the strip wells coated with an anti-acetyl H3-K27 antibody. The captured acetyl histone H3-K27 can then be detected with a labeled detection antibody, followed by a color development reagent. The ratio of acetyl H3-K27 is proportional to the intensity of absorbance. The absolute amount of acetyl H3-K27 can be quantified by comparing to the standard control。

产品特点：

1·Quick and efficient procedure, which can be finished within 2.5 hours；

2·Innovative colorimetric assay without the need for radioactivity, electrophoresis, or chromatography ；

3.Specifically captures acetyl H3-K27 with the detection limit as low as 2 ng/well and detection range from 20 ng-1 µg/well of histone extracts；

4.The control is conveniently included for the quantification of the amount of acetyl H3-K27 ；

5.Strip microplate format makes the assay flexible: manual or high throughput；

6.Simple, reliable, and consistent assay conditions。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

产品名称	规格	操作手册	货号	询价
总组蛋白H3K27乙酰化定量检测试剂盒（比色法）	48次	PDF	A-P-4059-48
总组蛋白H3K27乙酰化定量检测试剂盒（比色法）	96次	PDF	A-P-4059-96
EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina）	12次	PDF	A-P-1056A-12
EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina）	24次	PDF	A-P-1056A-24
96孔DNA甲基化极速修饰试剂盒（磁珠法）	96次	英文PDF-中文PDF	A-P-1050-096
96孔DNA甲基化极速修饰试剂盒（磁珠法）	192次	英文PDF-中文PDF	A-P-1050-192
抗人C5a/C5a desArg单克隆抗体(克隆号2942)	100ug	PDF	C-HM2078
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记	100ug	PDF	C-HM2022F
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记	50ug	PDF	C-HM2008
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记	50ug	PDF	C-HM2006
抗小鼠TREM-2单克隆抗体(克隆号6E9)	100ug	PDF	C-HM1129F
m6A RNA甲基化定量检测试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-9005-96
RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-P-9003-050
EZ RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-R5001

表观遗传学产品全面解决方案列下：     

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1036-96
DNA羟甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1037-96
DNA甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	英文PDF--中文PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	英文PDF--中文PDF	A-P-1014B-96

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