Magna 细胞核交联（RIP）抽提试剂盒 货号：A-17-10520
Magna Nuclear RIP™ (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit
背景资料：Magna Nuclear RIP™ Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq)。
产品描述：Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Cross-linked can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. In contrast native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. For less well understood proteins and protein complexes often both approaches are used.
The kit described here is for the cross-linked approach. If a native approach is of interest please visit the product page for the Magna Nuclear RIP (Native) Kit, catalogue # 17-10522 or the EZ-Magna Nuclear RIP (Native) Kit, catalogue # 17-10523。
产品特点：
1·Generates cross-linked chromatin to allow analysis of a variety of chromatin-associated RNAs ；
2·Flexible, scalable input requirements: Recover RNA from millions of cells or as few as 5,000 cells ；
3.Magnetic protein A/G bead blend and optimized buffer system results in low backgrounds and high signal-to-noise ratios；
4.Suitable for analysis by RT-qPCR or RIP-seq；
5.Suitable for analysis by RT-qPCR or RIP-seq；
6.RNA Binding Protein Immunoprecipitation (RIP);
7.Magna Nuclear RIP (Cross-Linked) RNA-Binding Protein Immunoprecipitation Kit is designed for the analysis of chromatin associated RNA such lncRNAs, enhancer RNAs and miRNAs。
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| Magna 细胞核交联（RIP）抽提试剂盒 |
24次 |
PDF |
A-17-10520 |
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表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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A-P-1037-48 |
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| DNA甲基化定量试剂盒(比色法) |
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A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
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