Magna ChIP™染色质免疫沉淀试剂盒                                                      货号：A-17-10460

Magna ChIP™ HiSens Chromatin Immunoprecipitation Kit

背景资料：Chromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes。

产品描述：The Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating the need to purchase different kits for different antibody isotypes. The SCW Buffer is unique to the Magna ChIP HiSens kit and enables the use of a single buffer for multiple steps of the ChIP process (sonication, chromatin immunoprecipitation, and wash). The ChIP elution buffer provided with the HiSens kit has been formulated to allow analysis of enrichment by qPCR without additional clean-up steps for more rapid results。

产品特点：

1·Robust ChIP from as few as 10,000 to as many as 1,000,000 cells；

2·Protocols and reagents for generation of chromatin from range of input sample type；

3.Superior performance with variety of isotypes of either polyclonal or monoclonal antibodies；

4.Specialized buffer and bead formulations; lower backgrounds and higher fold enrichment ；

5.Single buffer system for sonication, chromatin IP, and wash；

6.Perform analysis of enrichment without additional purification after cross link reversal;

7.Compatible with all commonly used downstream analysis applications– qPCR, next generation sequencing, microarray。

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

产品名称	规格	操作手册	货号	询价
Magna ChIP™染色质免疫沉淀试剂盒	24次	PDF	A-17-10460
EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina）	12次	PDF	A-P-1056A-12
EpiNext 高灵敏重亚硫酸盐测序试剂盒（Illumina）	24次	PDF	A-P-1056A-24
96孔DNA甲基化极速修饰试剂盒（磁珠法）	96次	英文PDF-中文PDF	A-P-1050-096
96孔DNA甲基化极速修饰试剂盒（磁珠法）	192次	英文PDF-中文PDF	A-P-1050-192
抗人C5a/C5a desArg单克隆抗体(克隆号2942)	100ug	PDF	C-HM2078
抗人TNF-R II单克隆抗体(克隆号80M2),FITC标记	100ug	PDF	C-HM2022F
抗人TNF-R II单克隆抗体(克隆号MR2-1),生物素标记	50ug	PDF	C-HM2008
抗人TNF-R I单克隆抗体(克隆号MR1-2),生物素标记	50ug	PDF	C-HM2006
抗小鼠TREM-2单克隆抗体(克隆号6E9)	100ug	PDF	C-HM1129F
m6A RNA甲基化定量检测试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-9005-96
RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-P-9003-050
EZ RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-R5001

表观遗传学产品全面解决方案列下：     

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1036-96
DNA羟甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1037-96
DNA甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	英文PDF--中文PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	英文PDF--中文PDF	A-P-1014B-96

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