ProStain™蛋白定量试剂盒 货号：E-15001
ProStain™ Protein Quantification Kit
背景资料：ProStain™ simplifies the determination of cell extract protein concentrations by providing highly sensitive detection reagents that are easy to use. The dye used in ProStain is resistant to many commonly found contaminants in cell lysis buffers such as detergent and salt, which limit the usefulness of other protein quantification systems. And, the spectral properties of ProStain change greatly after it is bound, effectively eliminating any background from unbound dye (see Properties tab). These characteristics make the ProStain Kit a significant improvement over other detection systems.
产品描述：Determining the protein concentration of cell extracts is an essential step in many modern cell biology procedures, such as Western blot and ELISA. Not surprisingly, there are numerous photometric and fluorescent methods for quantifying cell extract protein levels. Photometric methods have traditionally been based either on intrinsic absorbance at 280 nm or on reagent-based methods such as the Bradford and Lowry assays. However, these classical approaches tend to be time consuming, have limited sensitivity and may be influenced by the presence of contaminating substances. Fluorescent-based detection methods, while generally more sensitive, have also tended to suffer from limitations, such as the requirement for toxic co-factors, high rates of hydrolysis or unsuitable spectral properties. The ProStain Kit offers significant improvements in sensitivity, assay robustness and convenience when compared with existing methods.
产品使用：Sirt1 activator. It suppressed TNFα release from THP-1 cells and stimulated adipogenesis in 3T3L1 cells (ref 1). SIRT1 stimulation by SirtAct leads to decreased tissue factor mediated thrombogenicity in mice (ref 2). It inhibited NFκB activation and reduced inflammatory cytokine production in lamina propria mononuclear cells from IBD patients (ref 3). It prevented and cured experimental Colitis in a mouse model (ref 3).
产品应用：.
Normalized absorption and emission spectra of free (solid, purple lines) and conjugated ProStain dye (dotted, copper lines) in phosphate buffer of pH 7.2. As free dye and bound dye absorb at different wavelengths (612 nm vs. 503 nm) and the quantum yield of bound dye is 50-fold greater than that of free dye, background from free dye is effectively eliminated.
| Dye State |
Absorption |
Emission |
ε L/(mol-cm) |
Quantum Yield |
| Free dye |
612 nm |
665 nm |
60,000 |
< 1% |
| Conjugated dye |
503 nm |
600 nm |
24,000 |
50% |
| Table 1: Properties of ProStain dye. |
文献参考：
“Monitoring of endothelial cell activation in experimental sepsis with a two-step cell culture model” bySchildbergeret al (2010) Innate Immunity 16(5):278-287.
“Non-apoptotic Fas-induced regulation of cytokines in undifferentiated and decidualized human endometrial stromal cells depends on caspase-activity” by Fluhr et al (2011) Mol. Hum. Reprod. 17(2):127-134.
“Determination of Drug Toxicity Using 2D Speroids Constructed from an Immortal Human Hepatocyte cell Line” byFey et al (2012) Toxicol. Sci. 127(2):403-411.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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