EuTc                                                                                                  货号：E-15003

Europium Tetracycline (EuTc)

背景资料：The EuTc-Hydrogen Peroxide Assay is based on a novel, Europium-based fluorescent reagent that is used to detect free or enzymatically produced H₂O₂. Europium tetracycline (EuTc) detects hydrogen peroxide in a fast and easy method, and without the need for additional enhancer solutions. This makes EuTc a sensitive reagent to monitor catalases, peroxidases or glucose-oxidase activities through in vitroenzymatic assays, or to study substances that inhibit or activate these enzymes.

产品描述：Hydrogen peroxide (H₂O₂) is a reactive metabolic by-product that is a key regulator in a number of oxidative stress-related states. Functioning through NFκB and other factors, hydroperoxide-mediated pathways have been linked to asthma, atherosclerosis, diabetic vasculopathy, osteoporosis, a number of neurodegenerative diseases and Down’s syndrome. Due to its implication in these many disease states, there is much interest in sensitive assays to monitor hydrogen peroxide in biochemical, clinical, as well as in environmental samples. In addition, there is much research on the enzymes that produce and eliminate hydrogen peroxide, and on their inhibitors.

产品使用：

Fast, 10-minute incubation time
Applicable to turbid samples
Functions at neutral pH
No need for enzymatic amplification
Large Stoke’s shift decreases interference of background fluorescence
Long decay time enables time-resolved, or time-gated, measurement

产品应用：.

Active Motif’s EuTc-Hydrogen Peroxide Assay is based on a novel fluorescent reagent that is used to detect free or enzymatically produced H₂O₂. Europium Tetracycline (EuTc) detects hydrogen peroxide in a fast and easy method, and without the need for additional enhancer solutions. This makes EuTc a sensitive reagent to monitor catalases, peroxidases or glucose-oxidase activities through in vitro enzymatic assays, or to study substances that inhibit or activate these enzymes.

文献参考：

“A Europium Ion-based luminescent sensing probe for hydrogen peroxide” by Wolfbeis et al (2002) Angewandte Chemie International Edition 41(23):4495-4498.
 
“Determination of the activity of catalase using a europium(III)-tetracycline-derived fluorescent substrate” by Wu et al (2003) Analytical Biochemistry 320:129-135.
 
“Detection of Hydrogen Peroxide in River Water via a Microplate Luminescence Assay with Time-Resolved (“Gated”) Detection” by Lei et al (2003) Microchim. Acta 143:269-274.
 
“Time-Resolved Luminescence Imaging of Hydrogen Peroxide Using Sensor Membranes in a Microwell Format” by Schaeferling et al (2003) Applied Spectroscopy 57(11):1386-1392.
 
“Time-resolved enzymatic determination of glucose using a fluorescent europium probe peroxide” by Wu et al(2004) Anal. Bioanal. Chem. 380:619-626.
 
“Nonenzymatic direct assay of Hydrogen Peroxide at neutral pH using the Eu3Tc Fluorescent Probe” by Duerkop et al (2005) Journal of Fluorescence 15(5):755-761.
 
“Enhancement of Europium Luminescence in Tetracycline-europium Complexes in the presence of urea Hydrogen peroxide” by da Silva et al (2005) Journal of Fluorescence 15(5):667-671.
 
“A Novel method for Time-Resolved Fluorimetric Determination and Imaging of the Activity of peroxidase, and Its Application to an Enzyme-Linked Immunosorbent Assay” by Lin et al (2006) Chemistry 12 (10):2730-2738.
 
“Detecting hydrogen peroxide in leaves in vivo - a comparison of methods” by Snyrychova et al (209) Pysiologica Plantarum 135:1-18.

定购信息：      

产品名称	规格	操作手册	货号	询价
EuTc	1000次	PDF	E-15003
山竹醇-山竹子素	10mg	PDF	E-14077
炔丙基脲（HPA）	10mg	PDF	E-14035
染色的干细胞CDy1	50ul	PDF	E-14001
MODified™蛋白质结构域结合试剂盒	5 rxns	PDF	E-13007
MODified™组蛋白多肽分析试剂盒	5 arrays	PDF	E-13005
MODified™组蛋白多肽分析试剂盒	1 arrays	PDF	E-13001
重组 DNMT1 蛋白, active	10ug	PDF	E-31335
6-Methyladenosine (6-meA) 抗体 (pAb)	100ug	PDF	E-61495
6-Methyladenosine (6-meA) 抗体 (pAb)	10ug	PDF	E-61496
DNMT3A dimethyl Lys44 抗体 (pAb)	100ul	PDF	E-61323
DNMT3A dimethyl Lys44 抗体 (pAb)	10ul	PDF	E-61324
DNMT3A 抗体 (mAb)	200ug	PDF	E-39206-200
DNMT3A 抗体 (mAb)	100ug	PDF	E-39206-100
3-Methylcytosine (3-mC) 抗体	100ug	PDF	E-61111
3-Methylcytosine (3-mC) 抗体	10ug	PDF	E-61112
CRISPR/Cas9 单克隆抗体【7A9】	100ug	PDF	E-A-9000-100
CRISPR/Cas9 单克隆抗体【7A9】	50 ug	PDF	E-A-9000-50
CRISPR/Cas9 单克隆抗体【7A9】	10 ug	PDF	E-A-9000-10
CRISPR/Cas9 多克隆抗体	100ug	PDF	E-A-1111-100
CRISPR/Cas9 多克隆抗体	50 ug	PDF	E-A-1111-050
CRISPR/Cas9 多克隆抗体	10 ug	PDF	E-A-1111-010
APC标记大鼠抗小鼠CD90.2/Thy-1.2抗体	0.1mg	PDF	E-1750-11
PE标记仓鼠抗小鼠CD69/VEA抗体	0.2mg	PDF	E-1715-09L
低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体	0.5mg	PDF	E-1531-14
生物素标记山羊抗I型胶原蛋白抗体	0.2mg	PDF	E-1310-08
兔抗人DR5抗体	0.1mg	PDF	E-6600-01
SEPH标记山羊抗s-tag标签抗体	1.0ml	PDF	E-6500-25
抗小鼠TREM-2单克隆抗体(克隆号6E9)	100ug	PDF	C-HM1129F
96孔DNA甲基化极速修饰试剂盒（磁珠法）	192次	英文PDF--中文PDF	A-P-1050-192
m6A RNA甲基化定量检测试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-9005-96
RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-P-9003-050
EZ RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-R5001

表观遗传学产品全面解决方案列下：   

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1036-96
DNA羟甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1037-96
DNA甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	英文PDF--中文PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	英文PDF--中文PDF	A-P-1014B-96

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