Chromeo™活细胞线粒体染色试剂盒 货号：E-15005
Chromeo™ Live Cell Mitochondrial Staining Kit
背景资料：The Chromeo™ Live Cell Mitochondrial Staining Kit utilizes a proprietary, non-toxic, water-soluble, membrane-permeable dye that rapidly diffuses into live or fixed cells. In live cells it stains mitochondria with low background and little or no staining of other organelles. When fixed cells are stained, internal membranes and nucleoli become additional targets of the dye.
产品描述：The Chromeo™ Live Cell Mitochondrial Staining Kit features a unique dye that is not fluorescent until after it has reacted with an amino group, which induces a structural change that results in bright red fluorescence. The dye diffuses passively through the membrane of living cells, and localizes at the mitochondria where reaction and activation occur. The fluorescent dye shows similar properties in fixed cells. In addition to mitochondria, the kit stains intracellular membranes and nucleoli when the cells are first treated with fixation reagents. A long Stokes shift separates the excitation wavelength of 503 nm from the emission maximum at 610 nm. This long Stokes shift separates the fluorescence of the stain from any auto-fluorescence background of living cells. To selectively stain the nuclei of the cells, the blue fluorescent Hoechst stain is also included in the kit.
产品使用：Mitochondria play an important role within living cells as they are involved in a variety of cellular processes. In addition to their “main” function to generate cellular energy, mitochondria are involved in regulation of cell growth and differentiation; they also play a critical role in the induction of apoptosis, and are part of signaling cascades such as calcium signaling. The structural characteristics of mitochondria are variable and depend on the organism, the cell type and the metabolic state of the individual cell. Cell cycle-dependent changes in mitochondrial morphology have also been reported.
产品应用：.
Water soluble fluorescent live and fixed cell stain
No background fluorescence of the free dye
Low toxicity
Easy to use
Long Stokes shift eliminates background of cellular autofluorescence
Hoechst staining of the nucleus
Enables long-term labeling experiments
保存温度：
1 vial of lyophilized Chromeo™ Mitochondrial stain
1 vial of Solubilization Buffer
2 vials containing Hoechst Nuclear stain solution
Contents guaranteed 6 months when stored at -20°C in the dark.
The number of experiments will depend on the chosen concentration of the stain, which may vary between different cell types. The kit provides sufficient materials to stain up to 3000 wells in 8-well chambered slides, or up to 6000 wells in a 96-well plate.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
