Chromeo™LT活细胞膜染色试剂盒 货号：E-15007
Chromeo™ LT Live Cell Membrane Staining Kit
背景资料：The Chromeo™ LT Live Cell Membrane Staining Kit utilizes a proprietary, coumarin-based fluorescent complex* that rapidly diffuses into live or fixed cells and tissue. The stain accumulates in lipid structures such as plasma and internal membranes, lysosomes or lipid bodies, allowing them to be specifically labeled and imaged with low background. The use of Chromeo LT Live Cell Membrane Stain enables quick and easy staining of cellular lipid structures. Because it does not elicit toxicity, it is suitable for live cell applications. Its affinity for and retention in lipid structures and its stability towards photobleaching make it possible to monitor cells over a period of several days (see Figure 7 under the Application Data tab below).
产品描述：The Chromeo™ LT Live Cell Membrane Staining Kit features a cell permeable fluorescent complex that selectively stains lipid structures like plasma and internal membranes with high specificity. It can be used to stain membranes in living cells, in paraformaldehyde fixed cells and in Formalin-Fixed Paraffin-Embedded (FFPE) tissue. The fluorescent complex shows high specificity and low background staining, enabling the use of only nanomolar concentrations for efficient staining. There is no need to pre-treat or permeabilize the cells prior to the short incubation time of just 10 minutes. After only one wash step with PBS, the stained cells are ready for image acquisition. The stain can be excited from 420-490 nm, including the 488 nm argon laser line. Because the emission range is 500-540 nm, standard FITC filter sets can be used for image capture. These fluorescent properties enable the stain to be used in combination with blue fluorescent (e.g. Hoechst) or red fluorescent (e.g.Bodipy) dyes. In addition, because the stain is non-toxic and is retained by lipids, long-term labeling experiments lasting for several days can be performed.
产品使用：NRK cells were plated in 3 cm cell culture dishes and washed once with pre-warmed PBS before adding 30 nM Chromeo LT Live Cell Membrane staining solution. After incubation for 10 minutes at 37°C, cells were washed once with pre-warmed PBS and were imaged in PBS with a Nikon CLSM (60x objective).
产品应用：.
Water soluble fluorescent stain for live cells, for paraformaldehyde fixed cells and for tissue
Low background fluorescence
Low toxicity
Easy to use
Excitable at 488 nm
Use FITC filter sets
Long-term labeling experiments
保存温度：
4 vials of lyophilized fluorescent membrane stain. Each vial provides enough fluorescent complex for 30 ml of a 30 nM staining solution. Store at 4°C in the dark. Contents guaranteed 6 months when stored properly.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
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| DNA羟甲基化定量试剂盒(比色法) |
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| DNA羟甲基化定量试剂盒(荧光法) |
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A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
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| DNA甲基化定量试剂盒(荧光法) |
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A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
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| DNA甲基化定量试剂盒(比色法) |
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A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
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| 通用DNA甲基化定量试剂盒 |
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A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
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A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
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A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
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| 总DNA甲基化定量试剂盒 |
96 次 |
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