Chromeo™ 505山羊抗小鼠IgG 货号:E-15030
Chromeo™ 505 Goat anti-Mouse IgG
背景资料:Chromeo 505 Goat anti-Mouse IgG is recommended by Leica Microsystems forSuper-Resolution Fluorescence Microscopy using Leica's STimulated EmissionDepletion (STED) microscopes. It has also been tested and verified by Leica for use inGround State Depletion with Individual Molecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.
产品描述:Applications:
Immunofluorescence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution
Application: |
Immunofluorescence |
Absorption: |
514 nm |
Emission: |
530 nm |
Molar Extinction: |
70,000 M-1 cm-1 |
Quantum Yield: |
~30% |
|
Antibody Type: |
Polyclonal |
Isotype: |
IgG |
Host: |
Goat |
Reactivity: |
Mouse |
Purification: |
Affinity Purified |
|
产品使用:Contents: 1 mg (Catalog No. 15030) or 0.1 mg (Catalog No. 15050) of Chromeo™ 505 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.
Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.
Chromeo 505 can be excited in a range from 480-515 nm, which enables excitation by a green laser. Even if the emission spectrum is shifted towards longer wavelength by some nm, filters designed for fluorescein can be used with Chromeo 505 conjugated antibodies. In contrast to fluorescein, Chromeo 505 exhibits a large tolerance to a broad pH range and high photostability. The Chromeo 505 conjugated antibodies have absorption and emission maxima of approximately 514 and 530 nm, which are compatible with common excitation sources and filter sets.
产品应用:.
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
保存温度:
The conjugated antibody should be stored at 4°C protected from light.
定购信息:
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