Chromeo™ 488山羊抗小鼠IgG 货号:E-15031
Chromeo™ 488 Goat anti-Mouse IgG
背景资料:Chromeo 488 Goat anti-Mouse IgG is recommended by Leica Microsystems forSuper-Resolution Fluorescence Microscopy using Leica's STimulated EmissionDepletion (STED) microscopes. It has also been tested and verified by Leica for use inGround State Depletion with Individual Molecule return (GSDIM) using the SR GSD microscope. STED enables resolution of 50-70 nm, while GSDIM can mathematically re-construct images with resolution as low as 20 nm.
产品描述:Applications:
Immunofluorescence: 1:1,000 to 1:2,000 dilution
Plate-based assays: 1:800 to 1:1,000 dilution
Application: |
Immunofluorescence |
Absorption: |
498 nm |
Emission: |
524 nm |
Molar Extinction: |
73,000 M-1 cm-1 |
Quantum Yield: |
~48% |
|
Antibody Type: |
Polyclonal |
Isotype: |
IgG |
Host: |
Goat |
Reactivity: |
Mouse |
Purification: |
Affinity Purified |
|
产品使用:This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
产品应用:.
Contents: 1 mg (Catalog No. 15031) or 0.1 mg (Catalog No. 15051) of Chromeo™ 488 conjugated Goat anti-Mouse IgG (H+L) and 1 ml MAXfluor™ Mounting Medium. The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.
Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.
Chromeo 488 is spectrally similar to FITC and Alexa® 488. The conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching when combined with MAXfluor Mounting Medium. Chromeo 488 conjugated antibodies have absorption and emission maxima of approximately 490 and 527 nm, which are compatible with common excitation sources and filter sets.
温度保存:
The conjugated antibody and the MAXfluor Mounting Medium should be stored at 4°C protected from light.
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