Chromeo™ 494山羊抗小鼠IgG 货号：E-15052
Chromeo™ 494 Goat anti-Mouse IgG
背景资料：Contents: 1 mg (Catalog No. 15032) or 0.1 mg (Catalog No. 15052) of Chromeo™ 494 conjugated Goat anti-Mouse IgG (H+L). The antibody concentration is 2 mg/ml in 0.01 M potassium phosphate, 0.15 M sodium chloride pH 7.4, containing 2 mM sodium azide.
Specificity: This antibody was purified by immunoaffinity chromatography. It reacts with whole molecular mouse IgG and the light chains of other mouse immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins was observed.
Chromeo 494 is a unique dye with special spectral properties. It is excitable with a green laser and has a large Stokes shift of 134 nm. Chromeo 494 conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 494 and 628 nm, making them ideal partners for other 488 nm excitable dyes and conjugates.
产品描述：Applications:
Immunofluorescence: 1:100 to 1:250 dilution
Plate-based assays: 1:800 to 1:1,000 dilution
| Application: |
Immunofluorescence |
| Absorption: |
489 nm |
| Emission: |
624 nm |
| Molar Extinction: |
55,000 M-1 cm-1 |
| Quantum Yield: |
~7% |
|
| Antibody Type: |
Polyclonal |
| Isotype: |
IgG |
| Host: |
Goat |
| Reactivity: |
Mouse |
| Purification: |
Affinity Purified |
|
产品使用：To ensure a maximum in photostability of Chromeo 494 conjugates under all experimental conditions, we recommend the use of Mowiol or TDE as mounting medium. Vectashield or MAXfluor Mounting medium might negatively influence the photostability of Chromeo 494.
Chromeo 494 Goat anti-Mouse IgG is recommended by Leica Microsystems forSuper-Resolution Fluorescence Microscopy using Leica's STimulated EmissionDepletion (STED) microscopes. STED enables resolution of 50-70 nm.
温度保存：.
Store desiccated as supplied at -20°C for up to 2 years. Store solutions at -20°C for up to 3 months.
质量保证：
This product is guaranteed for 6 months from date of receipt.
This product is for research use only and is not for use in diagnostic procedures.
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