CE 染料 503 货号：E-15101
Capillary Electrophoresis (CE) Dye Kits
背景资料：The CE Dye Kits provide a convenient alternative for the fluorescent labeling of protein samples prior to capillary electrophoresis. Capillary electrophoresis (CE) has proven to be a useful tool in modern cell biology applications because it enables the rapid and high-resolution separation of proteins in a microsample format. These properties have made CE especially useful when performing single-cell protein analyses. However, traditional CE performed using UV absorbance can suffer from a lack of sensitivity due to the short path length across the capillary.
产品描述：CE Dyes overcome a large limitation associated with derivatization-based methods for labeling proteins because the positive charge of the amine group is maintained even after dye conjugation (Figure 1). This means that every protein labeled with CE Dye maintains its native charge, so you get single peak protein detection. With CE Dyes, there is no band broadening or need to perform adjusted ionic character calculations.
产品应用：CE Dye Kits provide a highly sensitive, convenient solution for capillary electrophoresis (CE). Upon binding to protein, CE Dyes undergo a large Stokes shift as well as a 50-fold increase in quantum yield. The result is sensitive detection with minimal background.
产品使用：
The CE Dye advantage
User-friendly, non-toxic labeling
Large Stokes shift for reduced background
Fast and simple procedure
Sensitive protein detection
文献参考：
CE Dyes have been successfully used as a stain in capillary electrophoresis. The detection limit in the picomolar range, the fast reaction time and the preservation of the protein’s native charge make our CE Dyes an interesting alternative to commonly used detection techniques.
“Determination of picomolar concentrations of protein using novel amino reactive chameleon labels and capillary electrophoresis laser-induced fluorescence detection” by Craig et al (2005) Electrophoresis 26(11):2208-2213.
“Reaction of organic reagents with proteins III. Spectroscopic and electrophoretic behavior of proteins labeled with Chromeo P503” by Turner et al (2008) J. of Chromatography 1194(2):253-256.
“Determination of biogenic amines by capillary electrophoresis using a chameleon type of fluorescent stain” bySteiner et al (2009) Microchimica Acta 167 (3-4):259-266.
“Polymer microchip CE of proteins either off- or on-chip labeled with chameleon dye for simplified analysis” by Yuet al (2009) Electrophoresis 30(24):4230-4236.
“Protein labeling Enhances Aptamer Selection by Methods of Capillary Electrophoresis” by de Jong et al (2011)Anal. Chem. 83:6330-6335.
“Preasure-Based Approach for the Analysis of Protein Adsorption in Capillary Electrophoresis; by de Jong et al(2012) Anal. Chem. 84:453-458.
“On-capillary fluorescent labeling and CE-LIF analysis of glycoforms of intact prostate-specific antigen; by Garrido-Medina et al (2013) Electophoresis 34(16):2295-2302.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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