MAXpack™封闭培养基 货号：E-15252
MAXblock™ Blocking Medium
背景资料：MAXblock™ Blocking Medium is a protein-based, non-mammalian blocking agent for use in immunofluorescence and immunostaining assays. Superior blocking is achieved utilizing a protein blend that demonstrates no cross-reactivity with secondary antibodies. It has been proven effective at blocking non-specific binding of antibodies in immunofluorescence, as well as in immunohistochemistry.
产品描述：Methanol-fixed HeLa cells grown on a coverslip were blocked overnight with MAXblock (A), 5% non-fat dry milk (C), 5% BSA (D) or Thermo-Fisher Pierce Sea Block (E). Cells were then incubated with a fluorescent anti-rabbit secondary antibody at a dilution of 1:250 (far above the recommended dilution to demonstrate the effectiveness of MAXblock), washed with MAXwash and mounted with DAPI. Any observed signal (pseudo-colored red) is caused by non-specific binding of the secondary due to incomplete blocking.
产品应用： Note that no staining is observed in panel A, demonstrating the effectiveness of MAXblock. (Panel B is a 150% overexposure of the fluorescent channel of the MAXblock slide, which shows cell position and demonstrates that antibody binding is barely detectable above background.) Experiments with an anti-mouse secondary gave nearly identical results.
产品使用：
For your convenience, MAXblock is also offered as a component in the MAXpack™ Immunostaining Media Kit, which also contains MAXbind™ Staining Medium and MAXwash™ Washing Medium.
温度保存：
150 ml of MAXblock Blocking Medium. The MAXpack™ Immunostaining Media Kit contains 150 ml MAXblock, 250 ml ofMAXbind™ and 1000 ml of MAXwash™. Store all media at 4°C; all reagents are guaranteed stable for 6 months when stored properly.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
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| DNA羟甲基化定量试剂盒(荧光法) |
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A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
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A-P-1037-96 |
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| DNA甲基化定量试剂盒(荧光法) |
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A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
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A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
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英文PDF--中文PDF |
A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
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| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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