Chromeo™ 494 NHS-Ester 货号：E-16111
Fluorescent Chromeo™ Dyes
背景资料：Chromeo™ Dyes – Chromeo 488, Chromeo 494, Chromeo 505, Chromeo 546 andChromeo 642 – are bright fluorescent labels that replace Alexa Fluor*, DyLight* or Cy*-dyes. They exhibit superior luminescence properties, broad Stokes shifts, stability towards photobleaching and pH, and low cell toxicity. In addition, they are compatible with most excitation sources including diode lasers, LEDs, tungsten lamps and xenon arc lamps. For your labeling convenience, Chromeo Dyes are offered in a variety of chemistries, as well as conjugated to secondary antibodies.
产品描述：To ensure that you get the best results possible, we highly recommend that you use Chromeo™ Dyes together with our MAX Stain™ Immunofluorescence Tools, as those components have been formulated to optimize the performance of the Chromeo™ Dyes.
产品应用：Reactive fluorescent Chromeo Dyes serve as bright labels for antibodies and other biomolecules, enabling detection in immunofluorescence, high content screening, ELISA, FRET applications or flow cytometry. For your convenience, Chromeo Dyes are also available as part of our easy-to-use Fluorescent Antibody Labeling Kits, and are offered as optimized Fluorescent Secondary Antibody Conjugates for enhanced detection.
产品使用：
Chromeo™ Dye advantages
High intensity
Photostability – the combination with MAX Stain™ reagents provides optimal fluorescent stability for multiple exposures and increased exposure time
Room temperature incubations
pH insensitive
Low background
Easy to use – no harsh chemicals required for conjugation
Because of their unique features, Chromeo Py-Dyes (Chromeo P429, P456, P503, P540 and P543) have been used successfully in a number of “no-wash” applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. See the Product Citations tab for a sample of published references.
温度保存：
To receive more detailed information and application data about individual Chromeo dyes, simply click on the name of the Chromeo dye of your choice in the table below. To see a larger spectra image, click on the spectrum of your choice.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
