Chromeo™ P543                                                                                     货号：E-16109

Fluorescent Chromeo™ Py-Dyes

背景资料：Chromeo Py-Dyes have been shown to work efficiently and with high sensitivity in different methods of protein electrophoresis. Proteins labeled with pyrylium dyesmaintain their native charge and isoelectric points, which results in sharp and characteristic bands during electrophoresis. For your convenience, Chromeo Py-Dyes are also included in our complete Capillary Electrophoresis (CE) Dye Kits, containing optimized and quality-controlled buffer systems.

产品描述：Chromeo™ Py-Dyes are a unique class of amine-reactive pyrylium dyes that change their color and become fluorescent upon reacting with primary amines. The shift in absorption and the induction of fluorescence effectively eliminate background effects from any unbound dye. The labeled proteins are ready to use immediately, and as the labeling method is fast, easy & reproducible, Chromeo Py-Dyes are suitable for a variety of “no-wash” applications:

Pre- and post-stain in SDS-protein electrophoresis
Pre-stain in capillary electrophoresis (CE)
Pre-stain in capillary isoelectric focusing (IEF)
Pre-stain in 2D capillary electrophoresis
Label for carbohydrate characterization
Small size label of proteins
Reagent to monitor amino functionalities on various surfaces

产品应用：Pyrylium dyes change their color and fluorescent properties upon reacting with proteins. After binding to primary amines, they undergo a large shortwave spectral shift that is visible to the naked eye (Figure 1). There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate. (See the Properties tab for detailed information on each Py-Dye).

产品使用：

Chromeo™ Py-Dye advantages

No washing steps required
No background from unbound dye
Fast and simple procedure
Small size
No change in net charge of the labeled molecule
No interference of excitation and emission wavelength

Because of their unique features, Chromeo Py-Dyes (Chromeo P429, P456, P503, P540 and P543) have been used successfully in a number of “no-wash” applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. See the Product Citations tab for a sample of published references.

文献参考：

Amine-reactive pyrylium dyes have been used as a pre-stain in protein electrophoresis. The high sensitivity with a detection-limit of 16 picograms and the fast, reproducible procedure improve electrophoresis results compared to other commercially available stains like SYPRO Red or silver staining.

“SDS-PAGE of proteins using a chameleon-type of fluorescence prestain” by Meier et al (2008) Anal. Chemistry80(16):6274-6279.
 
“Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli” by Maertens et al (2010) Protein Science 19:1312-1326.

定购信息：      

产品名称	规格	操作手册	货号	询价
Chromeo™ P543	5*1mg	PDF	E-16109
Chromeo™ 546山羊抗兔IgG，样品	100ug	PDF	E-15063
山竹醇-山竹子素	10mg	PDF	E-14077
炔丙基脲（HPA）	10mg	PDF	E-14035
染色的干细胞CDy1	50ul	PDF	E-14001
MODified™蛋白质结构域结合试剂盒	5 rxns	PDF	E-13007
MODified™组蛋白多肽分析试剂盒	5 arrays	PDF	E-13005
MODified™组蛋白多肽分析试剂盒	1 arrays	PDF	E-13001
重组 DNMT1 蛋白, active	10ug	PDF	E-31335
6-Methyladenosine (6-meA) 抗体 (pAb)	100ug	PDF	E-61495
6-Methyladenosine (6-meA) 抗体 (pAb)	10ug	PDF	E-61496
DNMT3A dimethyl Lys44 抗体 (pAb)	100ul	PDF	E-61323
DNMT3A dimethyl Lys44 抗体 (pAb)	10ul	PDF	E-61324
DNMT3A 抗体 (mAb)	200ug	PDF	E-39206-200
DNMT3A 抗体 (mAb)	100ug	PDF	E-39206-100
3-Methylcytosine (3-mC) 抗体	100ug	PDF	E-61111
3-Methylcytosine (3-mC) 抗体	10ug	PDF	E-61112
CRISPR/Cas9 单克隆抗体【7A9】	100ug	PDF	E-A-9000-100
CRISPR/Cas9 单克隆抗体【7A9】	50 ug	PDF	E-A-9000-50
CRISPR/Cas9 单克隆抗体【7A9】	10 ug	PDF	E-A-9000-10
CRISPR/Cas9 多克隆抗体	100ug	PDF	E-A-1111-100
CRISPR/Cas9 多克隆抗体	50 ug	PDF	E-A-1111-050
CRISPR/Cas9 多克隆抗体	10 ug	PDF	E-A-1111-010
APC标记大鼠抗小鼠CD90.2/Thy-1.2抗体	0.1mg	PDF	E-1750-11
PE标记仓鼠抗小鼠CD69/VEA抗体	0.2mg	PDF	E-1715-09L
低内毒素/无叠氮钠仓鼠抗小鼠CD3e抗体	0.5mg	PDF	E-1531-14
生物素标记山羊抗I型胶原蛋白抗体	0.2mg	PDF	E-1310-08
兔抗人DR5抗体	0.1mg	PDF	E-6600-01
SEPH标记山羊抗s-tag标签抗体	1.0ml	PDF	E-6500-25
抗小鼠TREM-2单克隆抗体(克隆号6E9)	100ug	PDF	C-HM1129F
96孔DNA甲基化极速修饰试剂盒（磁珠法）	192次	英文PDF--中文PDF	A-P-1050-192
m6A RNA甲基化定量检测试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-9005-96
RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-P-9003-050
EZ RNA甲基化修饰试剂盒	50 次	英文PDF--中文PDF	A-R5001

表观遗传学产品全面解决方案列下：   

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1036-96
DNA羟甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1037-96
DNA甲基化定量试剂盒(荧光法)	48 次	英文PDF--中文PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	英文PDF--中文PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	英文PDF--中文PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	英文PDF--中文PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	英文PDF--中文PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	英文PDF--中文PDF	A-P-1014B-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。