Chromeo™ P540 货号：E-16107
Fluorescent Chromeo™ Py-Dyes
背景资料：Chromeo Py-Dyes have been shown to work efficiently and with high sensitivity in different methods of protein electrophoresis. Proteins labeled with pyrylium dyesmaintain their native charge and isoelectric points, which results in sharp and characteristic bands during electrophoresis. For your convenience, Chromeo Py-Dyes are also included in our complete Capillary Electrophoresis (CE) Dye Kits, containing optimized and quality-controlled buffer systems.
产品描述：Chromeo™ Py-Dyes are a unique class of amine-reactive pyrylium dyes that change their color and become fluorescent upon reacting with primary amines. The shift in absorption and the induction of fluorescence effectively eliminate background effects from any unbound dye. The labeled proteins are ready to use immediately, and as the labeling method is fast, easy & reproducible, Chromeo Py-Dyes are suitable for a variety of “no-wash” applications:
Pre- and post-stain in SDS-protein electrophoresis
Pre-stain in capillary electrophoresis (CE)
Pre-stain in capillary isoelectric focusing (IEF)
Pre-stain in 2D capillary electrophoresis
Label for carbohydrate characterization
Small size label of proteins
Reagent to monitor amino functionalities on various surfaces
产品应用：Pyrylium dyes change their color and fluorescent properties upon reacting with proteins. After binding to primary amines, they undergo a large shortwave spectral shift that is visible to the naked eye (Figure 1). There is also a large increase in quantum yield that changes the non- or only weakly fluorescent dye into a bright fluorescent conjugate. (See the Properties tab for detailed information on each Py-Dye).
产品使用：
Chromeo™ Py-Dye advantages
No washing steps required
No background from unbound dye
Fast and simple procedure
Small size
No change in net charge of the labeled molecule
No interference of excitation and emission wavelength
Because of their unique features, Chromeo Py-Dyes (Chromeo P429, P456, P503, P540 and P543) have been used successfully in a number of “no-wash” applications such capillary electrophoresis, SDS-protein gel electrophoresis, isoelectric focusing or as a label in receptor binding studies. See the Product Citations tab for a sample of published references.
文献参考：
Amine-reactive pyrylium dyes have been used as a pre-stain in protein electrophoresis. The high sensitivity with a detection-limit of 16 picograms and the fast, reproducible procedure improve electrophoresis results compared to other commercially available stains like SYPRO Red or silver staining.
“SDS-PAGE of proteins using a chameleon-type of fluorescence prestain” by Meier et al (2008) Anal. Chemistry80(16):6274-6279.
“Gene optimization mechanisms: A multi-gene study reveals a high success rate of full-length human proteins expressed in Escherichia coli” by Maertens et al (2010) Protein Science 19:1312-1326.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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