重组 组蛋白 生物素化-H3.3 货号：E-31897
Recombinant Histone H3.3 - biotinylated
背景资料：Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker protein, present at the interface between the nucleosome core and DNA entry/exit points.
Histone H3.1 and Histone H3.3 are the two main Histone H3 variants found in plants and animals. They are known to be important for gene regulation. Histone H3.1 and H3.3 have been shown to demonstrate unique genomic localization patterns thought to be associated with their specific functions in regulation of gene activity. Specifically, Histone H3.1 localization is found to coincide with genomic regions containing chromatin repressive marks (H3K9me3, H3K27me3 and DNA methylation), whereas Histone H3.3 primarily colocalizes with marks associated with gene activation (H3K4me3,H2BK120ub1, and RNA pol II occupancy). Deposition of the Histone H3.1 variant into the nucleosome correlates with the canonical DNA synthesis-dependent deposition pathway, whereas Histone H3.3 primarily serves as the replacement Histone H3 variant outside of S-phase, such as during gene transcription. Aberrant localization of these variants is also known to correlate with certain cancers.
产品描述：
Recombinant Histone H3.3 - biotinylated, Human, corresponds to the native histone sequence of mammalian histone H3 variant H3.3 and does not contain any amino acid substitutions or residue analogs. Histone H3.3 is known to be important for gene regulation. Recombinant Histone H3.3 - biotinylated, Human consists of the full-length sequence of human Histone H3.3 variant (accession number NM_005324) and contains a C-terminal Biotin-tag. Recombinant Histone H3.3 - biotinylated, Human was generated in E. coli cells and has an observed molecular weight of 18 kDa. The recombinant protein is >70% pure by SDS-PAGE.
产品应用：
Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
产品使用：
100 µg Recombinant Histone H3.3 - biotinylated is supplied at a concentration of 0.4 µg/µl in 25 mM Tris-HCl, pH 7.5, 150 mM NaCl and 5% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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