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重组 ERK2 蛋白，带活性的 (E-31153) |
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重组 ERK2 蛋白，带活性的 货号：E-31153
Recombinant ERK2 protein
背景资料：The extracellular signal-regulated kinases 1 and 2 (ERK1 and ERK2), also called p44 and p42 MAP kinases, are members of the Mitogen Activated Protein Kinase (MAPK) family of proteins found in all eukaryotes. Because the 44 kDa ERK1 and the 42 kDa ERK2 are highly homologous and both function in the same protein kinase cascade, the two proteins are often referred to collectively as ERK1/2 or p44/p42 MAP kinase. The ERK1/2 signaling cascade has been shown to be a critical regulator of cell differentiation, cell physiology and neuronal function. Aberrant control of ERK1/2 activity has been implicated in a variety of pathological conditions, including cancer and autoimmune diseases, and efficient study methods are in demand.
产品描述：
Recombinant human ERK2 protein was expressed in E. coli as a N-His-fusion protein. The accession number is NM_002746. This highly active form was produced by phosphorylation of the purified protein in vitro with MEK1 and is suitable for labeling ERK2 substrates. This kinase is phosphorylated at Thr204. Purified by chromatography to > 95% by SDS-PAGE.
产品应用：
Recombinant ERK2 is suitable for kinase assays. The molecular weight of the protein is ~44.556 kDa. The activity of the protein is ~ 470,000 units/mg with 1 unit defined as the amount of enzyme that will catalyze the transfer of 1 pmol phosphate to myelin basic protein per minute at 30°C. Recommended Kinase Reaction Buffer: 20 mM Tris-HCL, 25 mM β-glycerophosphate, 5 mM EGTA, 1 mM sodium orthovanadate and 1 mM DTT, pH 7.5. Kinase activity may vary depending on the substrate and reaction conditions used.
产品使用：
10 µg of Recombinant CK2α2 protein in 20 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, 1 mM EDTA, pH 7.4. Protein is supplied at 1.9 µg/µl
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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