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| 精彩产品>免疫生物学...E... |
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重组 ER 蛋白 货号：E-31119
Recombinant ER protein
背景资料：Estrogen receptors (ER) are nuclear hormone receptors that mediate the actions of the endogenous steroid hormone, 17β-estradiol (E2). ER is synthesized as two protein forms, ERα and ERβ, which can bind to the estrogen receptor element (ERE) as homo- or heterodimers. Cell proliferation and differentiation triggered by ER activation has been heavily studied in bone-wasting and breast cancer treatments. Estrogen receptors are necessary for sexual development and reproductive function.
产品描述：Recombinant ER is suitable for DNA and protein-protein interaction assays. 20-100 ng are sufficient for in vitro transcription assays and 100 ng are sufficient for protein-protein interaction studies. The molecular weight of the protein is ~64 kDa. This protein is not recommended for use in TransAM®. NOTE: The presence of Poly [d(I-C)] in buffers may affect protein functionality and should be avoided.
产品应用：
Recombinant ER is specific for the ER alpha subunit and was expressed from full length (accession number NM 000125) with an amino terminal polyhistidine tag in a baculovirus system and purified by an affinity column in combination with FPLC chromatography. The purified recombinant protein is greater than 90% homogeneous and contains no detectable protease, DNase and RNase activity.
产品使用：
4 µg of Recombinant ER protein and Dilution Buffer AM1 (20 mM Tris-Cl (pH 8), 20% glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA). Protein is supplied at 0.2 µg/µl in Dilution Buffer AM1.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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