mTRAP™ 裂解缓冲液 货号:E-29011
mTRAP™ Components
背景资料:
Streptavidin Beads
Streptavidin Beads are stable, uniform, non-porous spheres bound with highly active streptavidin. They are RNase and DNase free for direct addition to cell or tissue lysates. The one-micron diameter beads bind 10 pmol biotinylated PNA-analog probe per 1 µl of beads and are supplied at ~1% solids in 50 mM Tris, pH 8, 150 mM NaCl, 0.05% sodium azide and 0.1% stabilizing detergent.
产品描述:
Streptavidin Beads
Streptavidin Beads are stable, uniform, non-porous spheres bound with highly active streptavidin. They are RNase and DNase free for direct addition to cell or tissue lysates. The one-micron diameter beads bind 10 pmol biotinylated PNA-analog probe per 1 µl of beads and are supplied at ~1% solids in 50 mM Tris, pH 8, 150 mM NaCl, 0.05% sodium azide and 0.1% stabilizing detergent.
产品应用:
mTRAP™ Lysis Buffer
mTRAP Lysis Buffer consists of 200 mM Tris (pH 7.5), 200 mM NaCl, 500 mM GTC (Guanidine Isothiocyanate) and a proprietary mixture of detergents. Store at room temperature; stable for 6 months.
产品使用:
Protease
Protease is active in SDS, EDTA, urea and at high temperatures and is supplied lyophilized. Store at -20°C; stable for 6 months.
温度保存:
LightSwitch miRNA Mimics and Inhibitors are shipped as lyophilized pellets. The lyophilized miRNA products are stable at -20°C. After resuspension, the products can be stored at -80°C. For maximum stability, minimize the number of freeze-thaw cycles of the products in solution. All reagents are guaranteed stable for 6 months when stored properly.
定购信息:
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
|
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |