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mTRAP™ Maxi 货号：E-23506
mTRAP™ Maxi & Midi
背景资料：mTRAP™ Maxi & mTRAP™ Midi Kits isolate high-quality mRNA from mammalian cells and tissue samples. The kits utilize Active Motif’s Poly T gripNA™ Probe*, which has a higher affinity and specificity for mRNA than oligo dT. The result is more mRNA per sample, with much less ribosomal RNA and genomic DNA contamination.
产品描述：Peptide Nucleic Acids (PNAs) are DNA analogs in which the nucleotides are attached to an N-(2-aminoethyl)glycine backbone instead of to deoxyribose, as in DNA1-4. However, poor water solubility and a tendency to self-aggregate have limited the utility of traditional PNAs. Active Motif has overcome these shortcomings by developing negatively charged PNAs5-7, which have a number of properties that make them ideal for mRNA isolation.
产品应用：
PNA molecules bind to complementary nucleic acids with a much higher affinity than oligo dT. This enables the use of binding and wash buffers that contain significantly lower salt concentrations than conventional methods. These conditions effectively eliminate stem/loop structures, enabling the Poly T gripNA Probe to hybridize to the poly A tails on all mRNA molecules. The low-ionic conditions also remove contaminating nucleic acid and protein molecules that may have formed weak, non-specific interactions with the Poly T gripNA Probe. Consequently, mTRAP Kits provide higher yields of a more representative population of mRNA, with significantly reduced ribosomal RNA and protein contaminations (Figure 1).
产品使用：
Why mTRAP™ and gripNAs?
Isolates substantially more mRNA than oligo dT
Reduces ribosomal RNA and genomic DNA contamination
Captures mRNA with secondary structure
Purifies a more representative mRNA population
Includes TouchDown™ for precipitating small quantities of nucleic acids
文献参考：
1. Nielsen P.E., et al. (1991) Science 254: 1497-1500.
2. Egholm M, et al. (1992) J. Am. Chem. Soc. 114: 1895-1897.
3. Harvey JC et al. (1992) Science 258: 1481-1485.
4. Buchardt O, et al. (1993) Trends Biotechnol. 11: 384-386.
5. Efimov VA, et al. (1998) NAR 26: 566-575.
6. van der Laan, et al. (1996) Tetrahedron Lett. 37: 7857-7860.
7. Efimov VA, et al. (1999) NAR 27: 4416-4426.
定购信息：
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