细胞有丝分裂分析试剂盒（Chemi） 货号：E-18022
Mitotic Assay Kits (Color & Chemi)
背景资料：As mitosis is the most defining stage of cell growth, Active Motif's chemiluminescent and colorimetric Mitotic Assay Kits are an easy way to test the effect of various treatments on cellular division. The kits utilize the same highly specific mitosis marker antibody as the fluorescent Mitotic Index Assay, but offer an alternative colorimetric or chemiluminescent readout, enabling you to choose whichever assay format best suits for your needs.
产品描述：Cell division is a complex, tightly regulated process that is marked by mitosis. Thus, accurate measurement of cells undergoing mitosis is a valuable method to characterize the effects of test compounds on cellular progression.
产品应用：
Two significant mitotic events include microtubule spindle formation and chromosome condensation. Histone H3 is phosphorylated on serine 28 during mitotic chromatin condensation before nuclear division occurs, making this event a reliable marker for cells undergoing mitosis.
产品使用：
Cells are grown in the included 96-well plates and then treated with whichever compound is being tested for its effect on cell growth. The cells are then fixed and incubated with the highly specific Histone H3 phospho Ser28 monoclonal antibody, followed by an HRP-conjugated secondary antibody for detection. Development is then performed with either colorimetric or chemiluminescent developing reagents. Paclitaxel, which arrests cells in late G2/M phase, is included to create a high-mitotic reference population. Cells can be counted and normalized using the included Crystal Violet stain.
温度保存：
Two 96-well plates for culturing cells, Histone H3 phospho Ser28 monoclonal antibody (Clone HTA28), HRP-conjugated secondary antibody, Paclitaxel, Quenching Solution, 1X Antibody Blocking Buffer, 1X Antibody Dilution Buffer, 10X PBS, 10% Triton X-100, 1% SDS Solution, Developing & Stop Solutions and Crystal Violet Cell Quantification Solution. Storage conditions vary from room temperature to -20°C, see manual for details. All reagents are guaranteed stable for 6 months when stored properly.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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| DNA羟甲基化定量试剂盒(荧光法) |
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A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
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| DNA甲基化定量试剂盒(荧光法) |
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英文PDF--中文PDF |
A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
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英文PDF--中文PDF |
A-P-1034-96 |
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| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
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| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
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