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重组 组蛋白 H2B 货号：E-31705
Recombinant Histone H2B
背景资料：Histone H2B is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 146 base pairs of DNA wrapped around an octamer of core histone proteins (two each of H2A, H2B, H3 and H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points.
产品描述：
Recombinant Xenopus laevis Histone H2B is produced in E. coli and purified using FPLC. Protein concentration was determined using the molar extinction coefficient for Histone H2B and absorbance at 280nm. The recombinant histone is >98% pure by SDS-PAGE. The molecular weight of the protein is 13,494 Daltons. The H2B protein is missing the first three residues (PEP).
The protein sequence is as follows: AKSAPA PKKGSKKAVT KTQKKDGKKR RKSRKESYAI YVYKVLKQVH PDTGISSKAM SIMNSFVNDV FERIAGEASR LAHYNKRSTI TSREIQTAVR LLLPGELAKH AVSEGTKAVT KYTSAK
Please click on the image below to view the mass spectrum.
产品应用：
Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro.
产品使用：
Recombinant Histone H2B is supplied as lyophilized powder. Recombinant histones can be resuspended in water or any suitable buffer. We recommend a starting concentration of 1 mg/ml. To fully solubilize the histone we suggest resuspension in the buffer of choice at room temperature for 20-30 minutes with occasional pipetting. Addition of salt or Tris to the resuspension buffer may enhance histone solubility.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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