重组 TBP 蛋白，带活性的 货号：E-31246
Recombinant TBP protein
背景资料：TBP (also known as TATA box binding protein, GTF2D, transcription initiation factor TFIID TBP subunit, TATA-binding factor, TF2D, TFIID or SCA17). RNA polymerase II is an enzyme found in eukaryotic cells composed of 12 subunits that catalyzes transcription of DNA. Transcription consists of three phases: initiation, elongation and termination. Transcription initiation involves TFIID, which is composed of TATA-binding protein (TBP) and TBP-associated factors (TAFs), as part of the RNA polymerase II pre-initiation complex (PIC). TBP binds to the TATA box sequence, which is located upstream of the transcription start site in some eukaryotic gene promoters, and helps position RNA polymerase II. TBP contains a string of glutamines at the N-terminus which affect DNA binding and the rate of transcription complex formation. TBP is also associated with RNA polymerase I and RNA polymerase III.
产品描述：
Recombinant TBP protein was expressed in E. coli as the full-length human protein (accession number NP_003185) with an N-terminal polyhistidine tag. The molecular weight of the protein is ~40 kDa.
产品应用：
Recombinant TBP protein is suitable for use in in vitro transcription assays. A recommended starting point is 1-5 ng recombinant protein per reaction.
产品使用：
10 µg of recombinant protein supplied at a concentration of 0.1 µg/µl in 20 mM Tris-HCl, pH 7.3, 100 mM KCl, 0.2 mM EDTA and 20% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
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