Elk-1 synthRE v1 货号：E-32123
LightSwitch™ Synthetic Response Elements
背景资料：LightSwitch™ Synthetic Response Element reporter constructs simplify the task of determining the effect that test compounds and/or conditions have on over 90 different transcription factors. Each synthRE vector contains an optimized synthetic response element, made up of repeats of a transcription factor binding site motif, cloned upstream of a minimal promoter and the RenSP luciferase gene in the Long-range Enhancer Reporter Vector, pLightSwitch_LR vector. As Synthetic Response Element vectors are supplied transfection-ready, you can begin your experiments immediately.
产品描述：
Similarly, for researchers studying post-transcriptional regulation events mediated by miRNA-3´UTR interactions, we offer a large collection of optimized, syntheticLightSwitch miRNA Target Reporters.
IMPORTANT: Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
Applications of Synthetic Response Elements:
Use as a primary screen to identify compounds or conditions that affect the activity of a biological pathway. Follow up using related endogenous promoter reporter constructs to detect subtle differences between compounds or conditions.
Generate dose response curves to various compounds or conditions.
Use as a positive control in your pathway-specific, cell-based assays.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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