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重组 SETDB1 蛋白 货号：E-31452
Recombinant SETDB1 protein
背景资料：SETDB1 (SET Domain, Bifurcated 1) is a histone methyltransferase that methylates histone H3 at lysine 9. H3K9 methylation is associated with transcriptional repression and gene silencing through recruitment of HP1 proteins to methylated histones. H3K9 trimethylation is coordinated with DNA methylation, most likely through formation of a complex with MBD1 and ATF7IP that leads to transcriptional repression. SETDB1 activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28, a factor recruited by KRAB zinc-finger proteins. Aberrant SETDB1 function is associated with the pathogenesis of Huntington Disease.
产品描述：
Recombinant SETDB1 (accession number NP_001138887.1) was expressed in Sf9 cells and contains an N-terminal FLAG tag with an observed molecular weight of 145 kDa. The recombinant protein is >85% pure by SDS-PAGE.
产品应用：
Recombinant SETDB1 is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.
Specific Activity: H3K9me3 methyltransferase.
HMT Assay Conditions: 30 ng/μl calf thymus Histone octamers were incubated with 30 ng/μl recombinant SETDB1 protein in reaction buffer including 50 mM TrisCl, pH 8.6, 0.02% Triton X-100, 2 mM MgCl2, 1 mM TCEP, 9 nCi/μl 3H-SAM for 2 hours at room temperature. Activity was detected by fluorography.
产品使用：
Full length recombinant SETDB1 protein expressed in Sf9 cells at a concentration of 0.6 mg/ml in 25 mM HEPES, pH 7.5, 120 mM NaCl, 0.2 mg/ml 3X FLAG peptide and 5% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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