 |
精彩产品 |
 |
|
|
|
|
|
 |
| 精彩产品>免疫生物学...E... |
| |
|
| |
| |
重组 BAZ1B (1340-1457) 蛋白 (E-31444) |
| |
|
|
重组 BAZ1B (1340-1457) 蛋白 货号：E-31444
Recombinant BAZ1B (1340-1457) protein
背景资料：Bromodomain adjacent to zinc finger domain, 1B (BAZ1B) is a member of the bromodomain protein family. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. BAZ1B is an atypical tyrosine protein kinase that plays a central role in chromatin remodeling and acts as a transcriptional regulator. It is an essential component of the WICH complex, a chromatin remodeling complex that mobilizes nucleosomes and reconfigures irregular chromatin to a regular nucleosomal array structure. The WICH complex regulates the transcription of various genes and has a role in RNA polymerase I and RNA polymerase III transcription. In the complex, BAZ1B mediates the recruitment of the WICH complex to replication foci during DNA replication. BAZ1B and the WICH complex are also involved in DNA damage response through phosphorylation of Tyr142 of histone H2AX (H2AXY142ph), a mark that distinguishes between apoptotic and repair responses to genotoxic stress. BAZ1B is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. In the WINAC complex, BAZ1B plays an essential role by targeting the complex to acetylated histones, an essential step for VDR-promoter association. Deletion of BAZ1B leads to Williams-Beuren syndrome.
产品描述：
The peptide corresponding to amino acids 1340-1457 that contains the bromodomain sequences of BAZ1B (accession number NP_115784.1) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 20.2 kDa. The recombinant protein is >90% pure by SDS-PAGE.
产品应用：
Recombinant BAZ1B (1340-1457) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
产品使用：
Recombinant BAZ1B (1340-1457) protein expressed in E. coli at a concentration of 4.7 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1036-48 |
 |
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1036-96 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1037-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
英文PDF--中文PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
英文PDF--中文PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
英文PDF--中文PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
英文PDF--中文PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
英文PDF--中文PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
英文PDF--中文PDF |
A-P-1014B-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
| |
|
|
| |
|
