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| 精彩产品>免疫生物学...E... |
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重组 BRWD3 (1295-1443) 蛋白 (E-31443) |
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重组 BRWD3 (1295-1443) 蛋白 货号：E-31443
Recombinant BRWD3 (1295-1443) protein
背景资料：Bromodomain and WD repeat domain containing 3 (BRWD3) is a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD) residues which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains a bromodomain and multiple WD repeats. The bromodomain functions as a ‘reader’ of epigenetic histone marks and regulates chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets. Mutations in BRWD3 cause mental retardation X-linked type 93, which is also referred to as mental retardation X-linked with macrocephaly. This gene is also associated with translocations in patients with B-cell chronic lymphocytic leukemia (B-CLL).
产品描述：
The peptide corresponding to amino acids 1295-1443 that contains the bromodomain sequences of BRWD3 (accession number NP_694984.4) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 23.37 kDa. The recombinant protein is >85% pure by SDS-PAGE.
产品应用：
Recombinant BRWD3 (1295-1443) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
产品使用：
100 µg of recombinant BRWD3 (1295-1443) protein expressed in E. coli at a concentration of 2.9 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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