重组 PRMT5 蛋白，带活性的 货号：E-31356
Recombinant PRMT5 complex
背景资料：PRMT5 (Protein Arginine Methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 Arg-3 during germ cell development. Methylates histone H3 Arg-8, which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR Tyr-1197 phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation. Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter.
产品描述：
N-terminally FLAG tagged PRMT5 (accession number NM_006109, MW=73 kDa, amino acids 2-end) were co-expressed with N-terminally His tagged MEP50 (accession number NM_024102, MW=37 kDa, amino acids 2-end) in Sf9 cells using a baculovirus expression system.
产品应用：
Recombinant PRMT5 complex is suitable for use in histone methyltransferase (HMT) assays.
Assay conditions: Prepare a 50 μl reaction mix (20 mM phosphate buffer pH 7.4, 20 μM S-adenosylmethionine, and 10-100 ng PRMT) and add to substrate-coated wells. Incubate for 1 hr at room temperature. Add primary antibody against methylated R3 residue of histone H4, incubate 1 hr. Next, incubate 30 minutes with the HRP-labeled secondary antibody followed by chemiluminescent detection.
产品使用：
20 µg recombinant PRMT5 complex expressed in Sf9 cells supplied in a buffer of 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ng/µl FLAG peptide and 20% glycerol at a concentration of 0.3 mg/ml.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
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