重组 GCN5 (726-837) 蛋白 货号：E-31371
Recombinant GCN5 (726-837) protein
背景资料：GCN5, also known as K (lysine) acetyltransferase 2A (KAT2A), is a component of the ATAC complex, which is a complex that specifically exerts histone acetyltransferase (HAT) activity on histones H3 and H4. GCN5 acts as a histone acetyltransferase to promote transcriptional activation. In the case of HIV-1 infection, it is recruited by the viral protein TAT and regulates TAT's transactivating activity. In addition, evidence suggests GCN5 may play a role in the induction of chromatin remodeling of proviral genes. GCN5 also functions as a repressor of NFκB by promoting ubiquitination of the NFκB subunit RelA in a HAT-independent manner. GCN5 contains a bromodomain that recognizes acetylated histone lysine residues and functions as a ‘reader’ of these epigenetic histone marks to regulate chromatin structure and gene expression by linking associated proteins to the recognized acetylated nucleosomal targets.
产品描述：
The peptide corresponding to amino acids 726 - 837 that contains the bromodomain sequences of GCN5 (accession number NM_021078.2) was expressed in E. coli and contains an N-terminal His tag and C-terminal FLAG tag with an observed molecular weight of 19.4 kDa. It shows binding specificity for acetylated H2AK5, H3K9, H3K14, H3K9/K14, H4K8/K16, H4K16 and H4K5/8/12/16.
产品应用：
Recombinant GCN5 (726-837) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
产品使用：
100 µg of recombinant GCN5 (726-837) protein expressed in E. coli at a concentration of 4 mg/ml in 25 mM HEPES pH 7.5, 150 mM NaCl and 5% glycerol.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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