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精彩产品>免疫生物学...E... |
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LightSwitch™ 最佳转染试剂盒 (E-32041) |
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LightSwitch™ 最佳转染试剂盒 货号:E-32041
LightSwitch™ Transfection Optimization Kit
背景资料:The LightSwitch™ Transfection Optimization Kit includes the key reagents and protocols needed to optimize transfection conditions for your cell line or condition of interest. The kit utilizes the FuGENE® HD Transfection Reagent, which is one of the most robust transfection reagents available, together with our LightSwitch™ Luciferase Assay Kitand LightSwitch Positive & Negative Control GoClone™ constructs.
产品描述:
The kit was designed to help you determine what our extensive experience with transfection and reporter assays has identified as the 3 key variables to successfully transfect a variety of different cell lines:
- FuGENE to plasmid ratio
- Cells seeded per well
- Duration of transfection
产品应用:
This product is for research use only and is not for use in diagnostic procedures.
产品使用:
The LightSwitch™ Transfection Optimization Kit contains:
LightSwitch™ Positive Control GoClone™ construct (-20°C)
LightSwitch™ Negative Control GoClone™ construct (-20°C)
FuGENE® HD Transfection Reagent (4°C)
LightSwitch™ Luciferase Assay Kit (100 assay size)
– Lyophilized 100X LightSwitch™ Assay Substrate (-20°C)
– LightSwitch™ Substrate Solvent (room temperature)
– LightSwitch™ Assay Buffer (-20°C)
Protocol
温度保存:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息:
表观遗传学产品全面解决方案列下:
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |
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