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海萤TK控制矢量 货号：E-32036
LightSwitch™ Dual Assay Kit
背景资料：The LightSwitch™ Dual Assay Kit was designed to normalize for variation between transfection replicates in cell lines that are particularly difficult to transfect by utilizing a co-transfection control plasmid. In addition to the reagents found in the LightSwitch Luciferase Assay Kit for detection of the RenSP luciferase found on LightSwitch Reporter vectors, the Dual Assay Kit includes reagents from the BioLux® Cypridina Luciferase Assay Kit developed by New England Biolabs for detecting the Cypridina luciferase produced from the Cypridina TK Control Vector (sold separately).
产品描述：
Because LightSwitch Reporter Vectors and the Cypridina TK Control Vector contain different luciferase genes that catalyze different substrates, there is no cross-reaction between the two signals. As an added advantage, the Cypridina reporter protein is secreted into the culture media while the RenSP reporter protein remains within the cell. Normalizing the LightSwitch signal using the Cypridina reporter signal may decrease variation between transfection replicates in difficult cell lines.
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
The LightSwitch™ Dual Assay Kit contains:
Lyophilized 100X LightSwitch™ Assay Substrate (-20°C)
LightSwitch™ Substrate Solvent (room temperature)
LightSwitch™ Assay Buffer (-20°C)
Lyophilized BioLux® Cypridina Luciferase Substrate (-20°C)
BioLux® Cypridina Luciferase Substrate Solvent (room temperature)
BioLux® Cypridina Luciferase Assay Buffer (-20°C)
Protocol
The kit does not include the Cypridina TK Control Vector.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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