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| 精彩产品>免疫生物学...E... |
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磷酸酶抑制剂 货号：E-37493
Phosphatase Inhibitor Cocktail
背景资料：Active Motif's Phosphatase Inhibitor Cocktail is designed to protect phosphoproteins from dephosphorylation during lysis and extraction procedures from mammalian cells or tissues and for immunoprecipitation and kinase assays. The cocktail is provided as a ready-to-use broad-spectrum solution of inhibitors targeting both serine/threonine phosphatases and protein tyrosine phosphatases (PTPs). The product is supplied as a 100X concentrated stock solution in a liquid format for improved accuracy, solubility, and ease of use in comparison to traditional tablets. To see the complete formulation, please click on the Description tab below.
产品描述：
The Phosphatase Inhibitor Cocktail is a broad-spectrum solution of phosphatase inhibitors specific for serine/threonine as well as protein tyrosine phosphatases (PTPs). It has been optimized and tested for use with mammalian cell and tissue extract samples. It is recommended to use 10 µl of the Phosphatase Inhibitor Cocktail solution to inhibit dephosphorylation of proteins for 1 ml of lysate. Since phosphatase levels may vary among cell and tissue types, it may be necessary to increase the concentration of inhibitors. The cocktail does not contain protease inhibitors. If necessary, protease inhibitors can be added to cell and tissue lysates. The inhibitor solution may not be compatible with immobilized metal chelate affinity chromatography and 2D gel electrophoresis. However, inhibitor components can be removed by dialysis or desalting prior to performing such procedures if necessitated
产品应用：
Broad-spectrum inhibition of dephosphorylation
Optimized for use with mammalian cell and tissue extracts
Convenient and stable liquid format for ease of use
产品使用：
Formulation:Sodium fluorideSodium orthovanadateSodium pyrophosphateGlycerophosphate
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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