脱乙酰酶抑制剂 货号：E-37494
Deacetylase Inhibitor
背景资料：ow you can easily purify histones and further separate the fractions of core histones from any cell culture or tissue sample while maintaining post-translational modifications like acetylation, methylation and phosphorylation states. The Histone Purification Kit enables you to isolate core histones from any cell culture or tissue sample. The core histones may be purified using the convenient spin column format as one total population containing H2A, H2B, H3 and H4, or further purified into separate fractions of H2A/H2B dimers and H3/H4 tetramers with the efficient gravity flow protocol.
产品描述：
To learn more about the Histone Purification Kit, click on the Description or Contentstabs below. To view a manual or other related documents, click on the Documents tab below.
产品应用：
How does it work?
Unlike histone purification by acid precipitation, our method utilizes a unique purification resin and a series of proprietary elution buffers to isolate very pure histone fractions. The resin has a high binding capacity for histones, so core histones can be isolated from small cell culture samples or from grams of tissue. Sequential elution steps let you collect the histones in either one step containing all core histones or into two separate populations: one enriched for H2A and H2B and a second fraction containing >90% pure H3 and H4 (Figure 1).
产品使用：
Histone Purification Kits include a reusable purification column and neutralization, equilibration, wash and H2A/H2B & H3/H4 elution buffers so you can easily purify core histones from your tissue or cell sample. The binding capacity of the purification resin is robust, so histones can be purified from as little as a single 100 mm plate, or from liters of culture or grams of tissue. The yield of core histones is about 0.4-0.5 mg of total core histones per 3 x 150 mm plates or 2.5 mg per gram of tissue, and the purified histones are ready for downstream analysis.
Core histones isolated by the Histone Purification Kit are highly pure and suitable substrates for downstream assays. The purified histones can also be used with our Chromatin Assembly Kit to enable the generation of chromatin that very closely resembles native chromatin for functional assays.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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