b-牛乳糖染色试剂盒 货号：E-35001
Chariot™ Protein Delivery Reagent
背景资料：Chariot™* is a revolutionary delivery reagent that quickly and efficiently transports biologically active proteins, peptides and antibodies directly into cells. The typical delivery efficiency is 60-95%. Less than two hours after delivery, live cells can be assayed to determine the effects of the introduced material, without the need for fixing. This makes Chariot an ideal tool for functional studies, including delivering inhibitory proteins, labeling organelles, screening peptide libraries and studying protein half-lives & transient complementation.
产品描述：
Chariot™ advantages
Works in a variety of cell lines (see table on the User Data tab)
Facilitates functional studies in living cells — no fixing required
Works in vivo1
Fast and efficient — 60-95% transfection in less than 2 hours
No need for expression of fusion proteins
Non-cytotoxic, serum independent
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
Why use Chariot™?
Current transfection techniques include cationic liposomes2, calcium phosphate coprecipitation3, electroporation4, microinjection5and viral vectors6. These methods are useful but are very time-consuming as once transfection is completed, it takes 12-80 hours to detect expression of the gene of interest. A greater drawback is that the cytotoxicity of these methods can make it difficult to determine if any observed changes are caused by the recombinant protein or induced by the transfection method itself.
Chariot is a novel transfection method that eliminates many of the problems encountered with more commonly used procedures. The Chariot peptide forms a non-covalent bond with the macromolecule of interest. This stabilizes the protein, protecting it from degradation and preserving its natural characteristics during the transfection process7, 8. After delivery, the complex dissociates, leaving the macromolecule biologically active and free to proceed to its target organelle. Delivery occurs in the presence or absence of serum and is independent of the endosomal pathway, which can modify macromolecules during internalization. This, coupled with the fact that Chariot is non-cytotoxic, ensures that observed changes are the result of your recombinant protein, and not the transfection method.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
