组蛋白H3 PTM多重试剂盒 货号：E-33115
Histone H3 PTM Multiplex Kit
背景资料：Active Motif's Histone H3 PTM Multiplex Assay is a novel high throughput bead-based ELISA assay designed for use with the MAGPIX®, Luminex® 200™ or FLEXMAP 3D® instruments to enable simultaneous interrogation of the levels of multiple histone H3 PTMs within the same sample. The 3-hour assay is ideally suited for high-throughput screening and profiling of histone modification levels of clinical or compound-treated samples. The multiplexing ability means you can simultaneously screen for on-target and off-target effects within the same sample. This kit offers several advantages over traditional methods (e.g. Western Blotting) including faster turnaround times and the ability to utilize smaller sample amounts.
产品描述：
Schematic of the Histone H3 PTM Multiplex Assay to study Histone H3 PTMs levels.
The 96-well plate-based assay works with nanogram quantities per well of acid extracted lysates or purified histones. The inclusion of a Histone H3 Total antibody bead set into the multiplex reaction enables normalization of Histone PTM levels across samples. To learn more about how the Histone PTM Multiplex Assay works or to read about the Luminex* technology, click on the Method tab below. To view data highlighting the advantages of the Histone PTM Multiplex Assay and its application, click on the Data tab. To view manuals, datasheets and other related documents, click on theDocuments tab.
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
How Do Histone H3 PTM Multiplex Kits Work?
The Histone H3 PTM Multiplex Assays work as bead-based "sandwich" assays to interrogate the levels of histone H3 modifications within acid extracted cell lysates or purified histones (see Figure 1). Samples are diluted in Assay Buffer and added to a 96-well assay plate. Histone post-translational modification antibodies, which are coupled to color-coded magnetic beads, are added to each well to capture the N-terminal histone H3 PTMs of interest in the sample. Using a 96-well magnetic plate, wells are washed to remove any unbound sample. A biotinylated Histone H3 antibody is added as a reporter to recognize the C-terminal domain of the histone samples bound to the antibody-conjugated beads. Following a wash step to remove unbound biotinylated antibody, streptavidin-phycoerythrin (SA-PE) is added to each well to bind the biotinylated reporter antibody. A final wash is performed to remove unbound SA-PE and the beads are resuspended in Wash Buffer and read using a MAGPIX™, Luminex®200™ or FLEXMAP 3D™ instrument and xPONENT® software. To view amanual for the Histone H3 PTM Multiplex assay, datasheets and other related documents, click on the Documents tab above.
Since each antibody-conjugated bead contains a different fluorescent label, antibodies for multiple histone PTMs can be added to the same sample for multiplexing. The readout from the streptavidin-phycoerythrin is proportional to the amount of histone PTM captured. A Histone H3 Total Ab-conjugated Bead set is available for normalization of PTM data across different samples. Active Motif offers the Histone H3 PTM Multiplex Kit and Histone Antibody-conjugated Beads to create your own custom multiplex or singleplex assays. All antibody-conjugate bead sets have been confirmed to work when multiplexed without any negative effects on assay results. A universal Assay Positive Control is also included to monitor the consistency of the performance of the assay between runs.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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