NFκB p65-结合磁珠 货号：E-33113
Luminex® Transcription Factor Assays
背景资料：® 200™ or MAGPIX® instruments. The Transcription Factor Multiplex Kits are novel bead-based assays designed to study analyte-specific DNA binding activity of proteins belonging to the same transcription factor family. Antibodies specific to each transcription factor protein are conjugated to a unique Luminex bead to enable comparative analysis of DNA binding between proteins in the same family. Kits are available for the study of AP-1 and NFκB transcription factor families.
产品描述：
Each Multiplex Kit contains a DNA probe of the transcription factor consensus binding sequence, competitor oligonucleotides, a positive control lysate, 96-well assay plate and the necessary buffers and detecting reagents to perform the assay. Antibody-conjugated beads must be purchased separately to complete the assay. The individual bead packaging allows researchers to customize their assays for singleplex or multiplex analysis simply by selecting antibody-conjugated beads for their protein(s) of interest. To learn more about how the Transcription Factor Multiplex Assays work or to read about the Luminex* technology, click on the Method tab below.
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
How Do Transcription Factor Multiplex Kits Work?
The Transcription Factor Multiplex Assays work as pull-down reactions where cell lysates are combined with a biotinylated double-stranded consensus binding sequence for the transcription factor family of interest. Fluorescently labeled magnetic beads, which have been conjugated to antibodies for the individual transcription factor family members, are used to capture the transcription factor-bound DNA. Streptavidin-phycoerythrin is used to bind the biotinylated oligo and determine the magnitude of signal (see Figure 1).
Since each antibody-conjugated bead contains a different fluorescent label, antibodies for multiple family members can be added to the same sample for multiplexing. The readout from the streptavidin-phycoerythrin is proportional to the amount of transcription factor binding. This allows for comparative analysis of DNA binding activity within the same transcription factor family. Active Motif offers buffer kits and antibody-conjugated magnetic beads to create your own custom multiplex or singleplex assays for the study of AP-1 and NFκB transcription factor families.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
