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| 精彩产品>免疫生物学...E... |
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NFkB v1 Reporter Cell Line (HT1080) (E-32214) |
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NFkB v1 Reporter Cell Line (HT1080) 货号：E-32214
LightSwitch™ Pathway Reporter Stable Cell Lines
背景资料：LightSwitch Pathway Reporter Stable Cell Lines simplify the task of measuring the response of specific pathways to treatment with various compounds or growth conditions. Each cell line contains a human regulatory element that has been cloned into a LightSwitch reporter vector and validated for use in measuring the activity of a specific biological pathway. Because they can be used in a variety of different plate-based formats, the Pathway Reporter Stable Cell Lines are ideal for both primary and secondary high-throughput screening.
产品描述：
If we don't have the stable cell line you need, Custom Services are available for creating custom stable cell lines, as well as for conducting screening services. Active Motif also offers LightSwitch Validated Pathway Collections, which are sets of promoter reporter constructs that showed significant activation or repression in response to various pathway-specific induction conditions.
IMPORTANT: Because all LightSwitch reporter constructs contain the optimized RenSP luciferase gene, you MUST use our LightSwitch Luciferase Assay Reagents to obtain optimal results. (Other luciferase assay reagents are not compatible with RenSP.)
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
Applications
Extended Assay Length – Measure changes in expression over longer time courses than are possible with transient assays.
High-throughput Screening – LightSwitch Pathway Reporter cell lines are ideal for both primary and secondary high-throughput screening, and can be used inn many different plate formats (96-, 384- and 1536-well plates).
Pathway Profiling – Use a panel of reporter cell lines to characterize the effect of your compounds on a variety of biological pathways.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
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