LigandLink™ pLL-1 共价试剂盒 货号：E-34007
LigandLink™ Universal Protein Labeling
背景资料：LigandLink™ Universal Labeling* enables specific in vivo, fluorescent labeling of your protein of interest. Simply express your protein as a LigandLink fusion, then add the LigandLink Label of your choice to the medium. To change the properties of the tag, just add a different LigandLink Label. This means that you only need to clone your gene once, but can label it with a variety of tags, depending on the needs of your experiment. For added convenience, the LigandLink Universal Labeling system is offered in both non-covalent and covalent designs.
产品描述：
Use of green fluorescent protein (GFP) and its derivatives has become commonplace in modern cell biology. However, biologically fluorescent proteins (FPs) are limited by their inherent properties. For example, FPs have a relatively low quantum yield, and it is difficult to engineer their spectral properties to suit specific applications.
Fluorescent dyes are a potential alternative to biological FPs as they are available in a broad variety of formats and can be engineered easily to ensure desirable spectral properties. However, because fluorescent dyes are synthetic molecules, it has not been possible to use them as a general tool for labeling specific proteins within a cell
产品应用：
This product is for research use only and is not for use in diagnostic procedures.
产品使用：
The eDHFR protein was chosen as a fusion partner because it is a relatively small, monomeric protein (18 kDa vs. 27 kDa for GFP) that has been shown to have a high affinity for the ligand trimethoprim (TMP). TMP binds with a high specificity to the E. coliform of DHFR (KI = ~1 nm), and a substantially lower affinity for endogenous DHFR (KI = ~4 µm). This is because TMP is an antibiotic that was designed to specifically inhibit the bacterial enzymes responsible for the production of folic acid while not interacting with mammalian proteins. The result is extremely low background, because there is minimal binding of LigandLink Labels to non-tagged mammalian proteins. Moreover, TMP can be derivatized to carry a number of tags without substantially altering its affinity and specificity for eDHFR.
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
