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精彩产品>免疫生物学...E... |
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F9 细胞核提取物 货号:E-36007
F9 nuclear extract
背景资料:F9 nuclear extract was prepared from cell cultures of the mouse teratocarcinoma F9 stem cell line. This cell line expresses characteristics of early mouse embryonal cells and is considered to be nullipotent because of its inability to spontaneously differentiate. However, introduction of chemical agents, such as retinoic acid (RA), or transfection with oncogenic sequences, such as SV40 large T antigen, can induce differentiation of these cells. F9 cells readily differentiate into an endodermal-like derivative phenotype, but possess the ability to differentiate into derivatives of all three germ layers under the appropriate culture conditions, suggesting that these cells may be pluripotent. The F9 stem cell line is widely used in research to study the biomolecular mechanisms underlying differentiation.
产品描述:
F9 nuclear extract is specifically recommended for studies related to differentiation and in stem cell research.
产品应用:
F9 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
产品使用:
Each lot has been tested for GATA-4 activation by using TransAM® Kits.
温度保存:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息:
表观遗传学产品全面解决方案列下:
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |
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