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精彩产品>免疫生物学...E... |
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人乳腺管癌细胞核提取物 货号:E-36027
T-47D nuclear extract
背景资料:T-47D nuclear extract was prepared from cell cultures of the human ductal breast epithelial tumor cell line. The T-47D cell line was derived from a pleural effusion of a 54-year old female patient with infiltrating ductal carcinoma. These cells exhibit a differentiated epithelial cell phenotype and express receptors to estradiol, as well as other steroids, and calcitonin. These cells also express mutant tumor suppressor p53and are therefore commonly used in research as in vitro models of mutant p53 breast cancer cells to study breast cancer tumorigenicity and related drug therapies, such as tamoxifen.
产品描述:
T-47D nuclear extract is specifically recommended for use in studies related to breast cancer and associated drug therapies.
产品应用:
T-47D nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
产品使用:
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
温度保存:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息:
表观遗传学产品全面解决方案列下:
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关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |
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