|
精彩产品 |
|
|
|
|
|
|
精彩产品>免疫生物学...E... |
|
|
|
|
SK-N-BE (2) 细胞核提取物 (E-36075) |
|
|
|
SK-N-BE (2) 细胞核提取物 货号:E-36075
SK-N-BE (2) nuclear extract
背景资料:SK-N-BE (2) nuclear extract was prepared from a cell culture of the human neuroblastoma SK-N-BE (2) cell line. The SK-N-BE (2) cell line was originally derived from a bone marrow biopsy of a 22 month old boy with disseminated neuroblastoma after cytotoxic therapy. The morphology of SK-N-BE (2) cells is variable, with some cells exhibiting epithelial-like morphology and others neuroblast-like morphology. The SK-N-BE (2) cell line is most commonly used in research related to differentiation, proliferation and apoptosis of neuroblast cells and as models for p53-mediated resistance to cytotoxic cancer therapies.
产品描述:
SK-N-BE (2) nuclear extract is recommended for use in studies related to differentiation, proliferation and apoptosis of neuroblast cells and as models for p53-mediated resistance to cytotoxic cancer therapies.
产品应用:
SK-N-BE (2) nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
产品使用:
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
温度保存:
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息:
表观遗传学产品全面解决方案列下:
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 |
|
|
|
|
|