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| 精彩产品>免疫生物学...E... |
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前列腺癌细胞核提取物 (睾丸素处理) (E-36067) |
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前列腺癌细胞核提取物 (睾丸素处理) 货号：E-36067
LNCaP nuclear extract (Testosterone treated)
背景资料：LNCaP nuclear extract (Testosterone treated) was prepared from cell cultures of the human prostate adenocarcinoma LNCaP cell line. The LNCaP cell line was originally derived from a supraclavicular lymph node metastatic lesion of a 50 year old Caucasian patient with metastatic prostate carcinoma. Prostate cancer is the second most common neoplasia in men. Because it is a hormone-responsive cancer, its treatment at initial stages of development typically involves anti-androgen adjuvant therapy. The fibroblast-like LNCaP cell line retains malignant properties that are characteristic of human prostate adenocarcinoma, including resistance to interferon, the expression of high levels of acid phosphatase, androgen receptors and Prostate Specific Antigen (PSA), and hormone responsiveness.
Treatment of LNCaP cells with testosterone and its biologically active metabolite dihydrotestosterone (DHT) stimulates cell growth and acid phosphatase production. The LNCaP cell line is most commonly used in research as a model for human prostate cancer.
产品描述：
LNCaP nuclear extract (Testosterone treated) is specifically recommended for studies related to prostate cancer.
产品应用：
LNCaP nuclear extract (Testosterone treated) is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 10 nM Testosterone for 6 hours at 37°C immediately prior to harvesting.
产品使用：
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
定购信息：
表观遗传学产品全面解决方案列下：
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