人宫颈癌细胞核提取物 货号：E-36038
HeLa S3 nuclear extract
背景资料：HeLa S3 nuclear extract was prepared from a cell culture of the HeLa S3 human epithelial carcinoma cell line. The HeLa S3 cell line is a subclone of the HeLa cell line that was originally derived from cells obtained in 1951 from Henrietta Lacks, a cervical cancer patient who eventually died from her cancer. The HeLa cell line is one of the oldest and most commonly utilized immortal cell lines in scientific research. These cells are highly prolific and easily sustainable, allowing for flexibility in their application in various fields of scientific research, including cancer biology, virology, and human disease. To date, over 60,000 publications across various disciplines have referenced the use of HeLa cells.
The HeLa S3 cell line has been adapted for growth in suspension (spinner) culture and demonstrates similar characteristics to the parent cell line, including its susceptibility to viral infection and propagation.
产品描述：
HeLa S3 nuclear extract is recommended for use in various applications across many research areas, including cancer, virology, and gene mapping studies. These cells are frequently utilized for analysis of clonal variation in relation to nutritional requirements, chromosome number, and plaque formation among mammalian cell populations.
产品应用：
HeLa S3 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl2, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).
产品使用：
Extracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
温度保存：
Lyophilized proteins can be stored at -20°C or -80°C, preferably desiccated. Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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