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ZR-96 Genomic DNA™ MagPrep |
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ZR-96 Genomic DNA™ MagPrep
About ZR-96 Genomic DNA™ MagPrep
The ZR-96 Genomic DNA™ MagPrep features a streamlined, high throughput (96-well) procedure for rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, solid tissue, bone marrow and buccal cells, cells from culture, and many biological liquid samples.
PCR inhibitors are effectively removed during the purification process. DNA purified using the ZR-96 Genomic DNA™MagPrep is suitable for PCR, nucleotide blotting, DNA sequencing, restriction endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
| DNA Recovery |
Up to 10 µg/well total DNA is eluted into ≥100 µl DNA Elution Buffer or water. Typical yields from samples are: 1-3 µg DNA per mg skeletal, heart, and brain tissues and 3-5 µg DNA per mg liver, kidney, and lung tissues. |
| Equipment |
Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually. |
| DNA Size Limits |
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources |
Solid tissues (e.g., tailsnips, earpunches, adipose tissue, etc.), whole blood, plasma,serum, buffy coat, lymphocytes, cultured cells, buccal cells, semen, tissues and other biological sources are effectively processed using this kit. |
| DNA Purity |
High-quality DNA is eluted with DNA Elution Buffer or water. DNA is especially well suited for PCR and other downstream applications. A260/A280>1.8 |
| Product Detergent Tolerance |
≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. |
Comparison between manual and automated processing.Porcine blood (50 µl) was sampled and DNA purification performed manually or using an automated liquid handling platform (e.g. Tecan Freedom EVO® 150 in this comparison). The comparison demonstrates the consistency of yield and recovered volumes afforded by the automated procedure.
Quick, high throughput (96-well) purification of DNA from whole blood, plasma, serum, body fluids, buffy coat,lymphocytes, swabs cultured cells, or tissues..
Combines Proteinase K digestion with magnetic bead purification
Isolated DNA is ideal for PCR, endonuclease digestion, bisulfite conversion/methylation detection, sequencing, genotyping, etc.
| ZR-96 Genomic DNA™ MagPrep |
2x96 |
D3083 |
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| ZR-96 Genomic DNA™ MagPrep |
4x96 |
D3084 |
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